Detailed information of TA system TA178188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 222611..223149
• Replicon: chromosome
• Accession: NZ_CP063421
• Organism: Vibrio cholerae strain VC_hf7

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: INP70_RS15085
• Protein ID: WP_000802134.1
• Coordinates: 222611..222910 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: INP70_RS15090
• Protein ID: WP_001107720.1
• Coordinates: 222907..223149 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP70_RS15055	218095..218484	+	390	WP_000491261.1	VOC family protein	-
INP70_RS15060	218643..219623	+	981	WP_000738239.1	DUF3883 domain-containing protein	-
INP70_RS15065	219917..220081	+	165	WP_001902694.1	DUF645 family protein	-
INP70_RS15070	220287..220775	+	489	WP_000110030.1	GNAT family N-acetyltransferase	-
INP70_RS15075	220911..221336	+	426	WP_000403004.1	GNAT family N-acetyltransferase	-
INP70_RS15080	221490..222494	+	1005	WP_000964930.1	LD-carboxypeptidase	-
INP70_RS15085	222611..222910	-	300	WP_000802134.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
INP70_RS15090	222907..223149	-	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
INP70_RS15095	223944..224375	+	432	WP_000795498.1	hypothetical protein	-
INP70_RS15100	224520..225034	+	515	Protein_246	GNAT family N-acetyltransferase	-
INP70_RS15105	225035..225151	+	117	WP_072612049.1	DUF3265 domain-containing protein	-
INP70_RS15110	225193..225969	+	777	WP_000758202.1	hypothetical protein	-
INP70_RS15115	226112..226429	-	318	WP_001180240.1	type II toxin-antitoxin system RelE/ParE family toxin	-
INP70_RS15120	226448..226690	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
INP70_RS15125	227082..227261	+	180	WP_032476891.1	DUF645 family protein	-
INP70_RS15130	227538..227828	+	291	WP_148529225.1	DUF3709 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	dfrA31	-	152411..247312	94901
	-	inside	Integron	-	-	153082..246602	93520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11601.34 Da
• Isoelectric Point: 9.9232

>T178188 WP_000802134.1 NZ_CP063421:c222910-222611 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQIGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T178188 NZ_CP083877:1810223-1810477 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATTATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT178188 WP_001107720.1 NZ_CP063421:c223149-222907 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT178188 NZ_CP083877:1809975-1810226 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A271VK51