Detailed information of TA system TA178187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 200758..201329
• Replicon: chromosome
• Accession: NZ_CP063421
• Organism: Vibrio cholerae strain VC_hf7

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A1T4SJW3
• Locus tag: INP70_RS14925
• Protein ID: WP_000351249.1
• Coordinates: 200928..201329 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q8GNG3
• Locus tag: INP70_RS14920
• Protein ID: WP_001080654.1
• Coordinates: 200758..200928 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INP70_RS14885	196257..196895	+	639	WP_000230121.1	HD domain-containing protein	-
INP70_RS14890	197070..197306	-	237	WP_000772125.1	RNA-binding protein	-
INP70_RS14895	197542..197823	+	282	WP_002023414.1	DUF1289 domain-containing protein	-
INP70_RS14900	198052..198714	+	663	WP_000084914.1	Vat family streptogramin A O-acetyltransferase	-
INP70_RS14905	198864..199247	+	384	WP_001081314.1	hypothetical protein	-
INP70_RS14910	199390..199878	+	489	WP_000424237.1	hypothetical protein	-
INP70_RS14915	200024..200562	+	539	Protein_209	hydrolase	-
INP70_RS14920	200758..200928	+	171	WP_001080654.1	hypothetical protein	Antitoxin
INP70_RS14925	200928..201329	+	402	WP_000351249.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
INP70_RS14930	201299..201442	+	144	WP_075042602.1	DUF3265 domain-containing protein	-
INP70_RS14935	201432..202067	+	636	WP_050547144.1	hypothetical protein	-
INP70_RS14940	202209..202745	+	537	WP_000644501.1	nucleotidyltransferase family protein	-
INP70_RS14945	202802..202885	+	84	WP_080099571.1	acetyltransferase	-
INP70_RS14950	202898..203257	+	360	Protein_216	VOC family protein	-
INP70_RS14955	203399..203833	+	435	WP_000453958.1	GNAT family N-acetyltransferase	-
INP70_RS14960	203923..204612	+	690	WP_193836708.1	FMN-binding negative transcriptional regulator	-
INP70_RS14965	204828..205745	+	918	WP_000186330.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	dfrA31	-	152411..247312	94901
	-	inside	Integron	-	-	153082..246602	93520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14636.77 Da
• Isoelectric Point: 4.0128

>T178187 WP_000351249.1 NZ_CP063421:200928-201329 [Vibrio cholerae]
MDIICFPFERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLA
VALEYLSLNDFELTQENDLLADAVRDLVIGIINETDFADILYAQYAKEQNSVL

Nucleotide

• Length: 402 bp

>T178187 NZ_CP083877:1809975-1810226 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6287.35 Da
• Isoelectric Point: 10.7274

>AT178187 WP_001080654.1 NZ_CP063421:200758-200928 [Vibrio cholerae]
MNRKVEAYGVDAVERPKIKASKKLDLTGDAGRQIVKSETKLALRTHQKTFTKLADM

Nucleotide

• Length: 171 bp

>AT178187 NZ_CP083877:1810223-1810477 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATTATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A1T4SJW3

Antitoxin

• AlphaFold DB: A0A1T4SJJ1