Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 187665..188163 Replicon chromosome
Accession NZ_CP063421
Organism Vibrio cholerae strain VC_hf7

Toxin (Protein)


Gene name - Uniprot ID Q9KMK7
Locus tag INP70_RS14805 Protein ID WP_000589156.1
Coordinates 187665..187931 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9KMK6
Locus tag INP70_RS14810 Protein ID WP_000643598.1
Coordinates 187918..188163 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INP70_RS14755 183228..183344 + 117 WP_080099589.1 DUF3265 domain-containing protein -
INP70_RS14760 183352..183669 - 318 WP_000077274.1 CcdB family protein -
INP70_RS14765 183669..183914 - 246 WP_001260801.1 type II toxin-antitoxin system CcdA family antitoxin -
INP70_RS14770 184111..184554 + 444 WP_148519660.1 hypothetical protein -
INP70_RS14775 184700..185176 + 477 WP_001047173.1 GNAT family N-acetyltransferase -
INP70_RS14780 185470..185598 + 129 WP_000947521.1 DUF645 family protein -
INP70_RS14785 185614..185652 + 39 WP_108244118.1 hypothetical protein -
INP70_RS14790 186395..186652 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
INP70_RS14795 186640..186942 + 303 WP_000229322.1 type II toxin-antitoxin system RelE/ParE family toxin -
INP70_RS14800 187102..187488 + 387 WP_001015082.1 MmcQ/YjbR family DNA-binding protein -
INP70_RS14805 187665..187931 + 267 WP_000589156.1 BrnT family toxin Toxin
INP70_RS14810 187918..188163 + 246 WP_000643598.1 hypothetical protein Antitoxin
INP70_RS14815 188304..188729 + 426 WP_002023452.1 GNAT family N-acetyltransferase -
INP70_RS14820 188848..189129 + 282 WP_002023460.1 DUF1289 domain-containing protein -
INP70_RS14825 189542..189713 + 172 Protein_191 DUF645 family protein -
INP70_RS14830 189913..190191 - 279 WP_000578475.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
INP70_RS14835 190188..190472 - 285 WP_000381184.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
INP70_RS14840 190826..190954 + 129 WP_000947521.1 DUF645 family protein -
INP70_RS14845 190970..191008 + 39 WP_108244118.1 hypothetical protein -
INP70_RS14850 191214..191525 + 312 WP_000623749.1 hypothetical protein -
INP70_RS14855 191653..191952 - 300 WP_000802134.1 type II toxin-antitoxin system RelE/ParE family toxin -
INP70_RS14860 191949..192191 - 243 WP_001107720.1 type II toxin-antitoxin system ParD family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island dfrA31 - 152411..247312 94901
- inside Integron - - 153082..246602 93520


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(6-81)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10092.34 Da        Isoelectric Point: 7.3171

>T178184 WP_000589156.1 NZ_CP063421:187665-187931 [Vibrio cholerae]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Download         Length: 267 bp

>T178184 NZ_CP083877:753749-754045 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9478.77 Da        Isoelectric Point: 6.4832

>AT178184 WP_000643598.1 NZ_CP063421:187918-188163 [Vibrio cholerae]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Download         Length: 246 bp

>AT178184 NZ_CP083877:754047-754442 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A271VMP7

Antitoxin

Source ID Structure
AlphaFold DB A0A271VME2

References