Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 173521..174287 Replicon chromosome
Accession NZ_CP063421
Organism Vibrio cholerae strain VC_hf7

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag INP70_RS14675 Protein ID WP_000982260.1
Coordinates 173521..174018 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0Q0PQR2
Locus tag INP70_RS14680 Protein ID WP_000246255.1
Coordinates 174015..174287 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INP70_RS14640 168726..169151 + 426 WP_001287987.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
INP70_RS14645 169457..169639 + 183 WP_000947620.1 DUF645 family protein -
INP70_RS14650 169924..170202 + 279 WP_000280315.1 DUF3709 domain-containing protein -
INP70_RS14655 170519..170932 + 414 WP_000049417.1 VOC family protein -
INP70_RS14660 171125..172111 - 987 WP_001170902.1 sulfate ABC transporter substrate-binding protein -
INP70_RS14665 172345..172740 + 396 WP_000046952.1 hypothetical protein -
INP70_RS14670 172891..173379 + 489 WP_000424237.1 hypothetical protein -
INP70_RS14675 173521..174018 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
INP70_RS14680 174015..174287 - 273 WP_000246255.1 DUF1778 domain-containing protein Antitoxin
INP70_RS14685 174439..174504 + 66 Protein_163 acetyltransferase -
INP70_RS14690 174840..176123 + 1284 WP_000097716.1 reverse transcriptase/maturase family protein -
INP70_RS14695 176307..176630 + 324 WP_080099580.1 DUF3709 domain-containing protein -
INP70_RS14700 176931..177302 + 372 WP_001008376.1 hypothetical protein -
INP70_RS14705 177619..177789 + 171 Protein_167 DUF645 family protein -
INP70_RS14710 177967..178248 + 282 WP_002023414.1 DUF1289 domain-containing protein -
INP70_RS14715 178505..179143 + 639 WP_000230121.1 HD domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island dfrA31 - 152411..247312 94901
- inside Integron - - 153082..246602 93520


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T178182 WP_000982260.1 NZ_CP063421:c174018-173521 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T178182 NZ_CP083877:230249-230356 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9713.24 Da        Isoelectric Point: 6.2988

>AT178182 WP_000246255.1 NZ_CP063421:c174287-174015 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT178182 NZ_CP083877:c230201-230135 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0Q0PQR2

References