Detailed information of TA system TA178178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1180113..1180774
• Replicon: chromosome
• Accession: NZ_CP063418
• Organism: Actinomyces sp. ZJ34

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HRL51_RS05035
• Protein ID: WP_172120741.1
• Coordinates: 1180349..1180774 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HRL51_RS05030
• Protein ID: WP_172120742.1
• Coordinates: 1180113..1180352 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRL51_RS05015	1175891..1178947	+	3057	WP_172120744.1	type I restriction endonuclease subunit R	-
HRL51_RS05020	1179014..1179215	-	202	Protein_970	hypothetical protein	-
HRL51_RS05025	1179506..1180021	+	516	WP_172193043.1	hypothetical protein	-
HRL51_RS05030	1180113..1180352	+	240	WP_172120742.1	Arc family DNA-binding protein	Antitoxin
HRL51_RS05035	1180349..1180774	+	426	WP_172120741.1	type II toxin-antitoxin system VapC family toxin	Toxin
HRL51_RS05040	1180771..1181160	-	390	WP_172120740.1	hypothetical protein	-
HRL51_RS05045	1181516..1182748	+	1233	WP_172120739.1	MFS transporter	-
HRL51_RS05050	1182912..1183799	-	888	WP_172120738.1	helix-turn-helix transcriptional regulator	-
HRL51_RS05055	1183951..1184568	+	618	WP_172120737.1	TetR family transcriptional regulator	-
HRL51_RS05060	1184669..1185490	+	822	WP_172120782.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (17-41)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15870.16 Da
• Isoelectric Point: 6.7323

>T178178 WP_172120741.1 NZ_CP063418:1180349-1180774 [Actinomyces sp. ZJ34]
VIVLDTNVLSEPLRRDPDPRVARWLDDQHPRTLYVTALTLAELRAGVEILPSGRRRDLLRERLEEEVIPFFEDRVLPFDG
RASRAWARLQAHARTHGRPLPVMDSLIAAVVSARGYALATRNTADFAATGVELINPWEAVV

Nucleotide

• Length: 426 bp

>T178178 NZ_CP083875:c74751-74602 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8810.84 Da
• Isoelectric Point: 5.1200

>AT178178 WP_172120742.1 NZ_CP063418:1180113-1180352 [Actinomyces sp. ZJ34]
MASVIVRNLTDDTHRALKARAHEHGRSMEAEIRAILDEAIEDQPRRGLGTLLSSIRQESGGADLEITRDRSVYEPLDLS

Nucleotide

• Length: 240 bp

>AT178178 NZ_CP083875:74795-74856 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT