Detailed information of TA system TA178174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 95029..95780
• Replicon: plasmid pKv8917
• Accession: NZ_CP063404
• Organism: Klebsiella variicola strain 8917

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: AMN10_RS26110
• Protein ID: WP_072046196.1
• Coordinates: 95029..95511 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: AMN10_RS26115
• Protein ID: WP_004902250.1
• Coordinates: 95502..95780 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMN10_RS26090	91427..92074	-	648	WP_014386537.1	EcsC family protein	-
AMN10_RS26095	92101..92856	-	756	WP_072046193.1	DUF2971 domain-containing protein	-
AMN10_RS26100	92895..93350	-	456	WP_136894309.1	hypothetical protein	-
AMN10_RS26105	93455..93994	-	540	WP_072046194.1	hypothetical protein	-
AMN10_RS26110	95029..95511	-	483	WP_072046196.1	GNAT family N-acetyltransferase	Toxin
AMN10_RS26115	95502..95780	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
AMN10_RS26120	95899..96111	-	213	WP_072046197.1	hypothetical protein	-
AMN10_RS26125	97466..97780	+	315	WP_017146616.1	hypothetical protein	-
AMN10_RS26130	98024..98506	-	483	WP_176694862.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..343094	343094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17678.45 Da
• Isoelectric Point: 8.9130

>T178174 WP_072046196.1 NZ_CP063404:c95511-95029 [Klebsiella variicola]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T178174 NZ_CP083872:3677398-3677676 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT178174 WP_004902250.1 NZ_CP063404:c95780-95502 [Klebsiella variicola]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT178174 NZ_CP083872:3677135-3677395 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3