Detailed information of TA system TA178173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 16880..17790
• Replicon: plasmid pKv8917
• Accession: NZ_CP063404
• Organism: Klebsiella variicola strain 8917

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: AMN10_RS25725
• Protein ID: WP_004144067.1
• Coordinates: 17320..17790 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: AMN10_RS25720
• Protein ID: WP_004026357.1
• Coordinates: 16880..17323 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMN10_RS25690	12163..12573	+	411	WP_072046160.1	hypothetical protein	-
AMN10_RS25695	12625..13113	+	489	WP_061891943.1	hypothetical protein	-
AMN10_RS25700	13198..13611	+	414	WP_072046161.1	NB-ARC domain protein	-
AMN10_RS25705	13673..14452	+	780	WP_072046162.1	hypothetical protein	-
AMN10_RS25710	15087..15509	+	423	WP_072046163.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
AMN10_RS25715	15509..16779	+	1271	Protein_22	translesion error-prone DNA polymerase V subunit UmuC	-
AMN10_RS25720	16880..17323	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
AMN10_RS25725	17320..17790	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
AMN10_RS25730	17900..18160	-	261	WP_004026352.1	hypothetical protein	-
AMN10_RS25735	18731..19912	-	1182	WP_193810132.1	IS481 family transposase	-
AMN10_RS25740	19982..20140	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
AMN10_RS25745	20519..20761	-	243	WP_072046223.1	hypothetical protein	-
AMN10_RS25750	20837..21001	-	165	WP_011154586.1	hypothetical protein	-
AMN10_RS25755	21118..21279	-	162	WP_164507110.1	hypothetical protein	-
AMN10_RS25760	21669..22643	+	975	WP_176694864.1	Hok/Gef family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..343094	343094
	-	flank	IS/Tn	-	-	18731..19912	1181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T178173 WP_004144067.1 NZ_CP063404:17320-17790 [Klebsiella variicola]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T178173 NZ_CP083872:c3671336-3670938 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT178173 WP_004026357.1 NZ_CP063404:16880-17323 [Klebsiella variicola]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT178173 NZ_CP083872:c3671632-3671339 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6P1V3Q9