Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4094343..4094962 Replicon chromosome
Accession NZ_CP063403
Organism Klebsiella variicola strain 8917

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag AMN10_RS20040 Protein ID WP_002892050.1
Coordinates 4094744..4094962 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag AMN10_RS20035 Protein ID WP_008805436.1
Coordinates 4094343..4094717 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMN10_RS20025 4089498..4090691 + 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AMN10_RS20030 4090714..4093860 + 3147 WP_064179935.1 multidrug efflux RND transporter permease subunit AcrB -
AMN10_RS20035 4094343..4094717 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
AMN10_RS20040 4094744..4094962 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
AMN10_RS20045 4095121..4095687 + 567 WP_022066286.1 maltose O-acetyltransferase -
AMN10_RS20050 4095659..4095787 - 129 Protein_3958 hypothetical protein -
AMN10_RS20055 4095824..4096294 + 471 WP_008805434.1 YlaC family protein -
AMN10_RS20060 4096263..4097720 - 1458 WP_072045890.1 PLP-dependent aminotransferase family protein -
AMN10_RS20065 4097821..4098519 + 699 WP_072045891.1 GNAT family N-acetyltransferase -
AMN10_RS20070 4098516..4098656 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
AMN10_RS20075 4098656..4098919 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T178172 WP_002892050.1 NZ_CP063403:4094744-4094962 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T178172 NZ_CP083872:3416480-3416698 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT178172 WP_008805436.1 NZ_CP063403:4094343-4094717 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT178172 NZ_CP083872:3416080-3416454 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References