Detailed information of TA system TA178138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5336921..5337561
• Replicon: chromosome
• Accession: NZ_CP063395
• Organism: Pseudomonas aeruginosa strain ST167_d57blood

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IPV39_RS24715
• Protein ID: WP_003134109.1
• Coordinates: 5336921..5337331 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV39_RS24720
• Protein ID: WP_031634724.1
• Coordinates: 5337331..5337561 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV39_RS24675	5332447..5333244	+	798	WP_235597565.1	hypothetical protein	-
IPV39_RS24680 (IPV39_24685)	5333401..5334129	+	729	WP_004352842.1	TIGR03761 family integrating conjugative element protein	-
IPV39_RS24685 (IPV39_24690)	5334135..5334683	+	549	WP_004352841.1	DUF3158 family protein	-
IPV39_RS24690 (IPV39_24695)	5334730..5335569	+	840	WP_003105750.1	Rha family transcriptional regulator	-
IPV39_RS24695 (IPV39_24700)	5335599..5336087	+	489	WP_004352840.1	single-stranded DNA-binding protein	-
IPV39_RS24700 (IPV39_24705)	5336243..5336410	+	168	WP_128729268.1	CrpP family ICE-associated protein	-
IPV39_RS24705 (IPV39_24710)	5336476..5336694	-	219	WP_023083218.1	hypothetical protein	-
IPV39_RS24710 (IPV39_24715)	5336708..5336905	-	198	WP_023083219.1	hypothetical protein	-
IPV39_RS24715 (IPV39_24720)	5336921..5337331	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IPV39_RS24720 (IPV39_24725)	5337331..5337561	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IPV39_RS24725 (IPV39_24730)	5337817..5339736	+	1920	WP_004352838.1	type I DNA topoisomerase	-
IPV39_RS24730 (IPV39_24735)	5340044..5340253	+	210	WP_003105733.1	cold-shock protein	-
IPV39_RS24735 (IPV39_24740)	5340474..5342363	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5318208..5407408	89200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T178138 WP_003134109.1 NZ_CP063395:c5337331-5336921 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T178138 NZ_CP083867:76072-76377 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT178138 WP_031634724.1 NZ_CP063395:c5337561-5337331 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT178138 NZ_CP083867:75852-76070 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA