Detailed information of TA system TA178124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3690510..3691731
• Replicon: chromosome
• Accession: NZ_CP063393
• Organism: Pseudomonas aeruginosa strain ST167_d67burn2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IPV41_RS16970
• Protein ID: WP_003160088.1
• Coordinates: 3691153..3691731 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV41_RS16965
• Protein ID: WP_003088112.1
• Coordinates: 3690510..3691064 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV41_RS16940 (IPV41_16945)	3686078..3686629	+	552	WP_003102727.1	XRE family transcriptional regulator	-
IPV41_RS16945 (IPV41_16950)	3686626..3687024	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IPV41_RS16950 (IPV41_16955)	3687037..3687360	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IPV41_RS16955 (IPV41_16960)	3687691..3688152	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IPV41_RS16960 (IPV41_16965)	3688157..3690352	+	2196	WP_034010345.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IPV41_RS16965 (IPV41_16970)	3690510..3691064	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
IPV41_RS16970 (IPV41_16975)	3691153..3691731	+	579	WP_003160088.1	HD domain-containing protein	Toxin
IPV41_RS16975 (IPV41_16980)	3691840..3693027	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IPV41_RS16980 (IPV41_16985)	3693017..3695188	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
IPV41_RS16985 (IPV41_16990)	3695178..3696263	-	1086	WP_243496770.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21816.65 Da
• Isoelectric Point: 5.1625

>T178124 WP_003160088.1 NZ_CP063393:3691153-3691731 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQCLAARRDA

Nucleotide

• Length: 579 bp

>T178124 NZ_CP083862:31714-32028 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAAACTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT178124 WP_003088112.1 NZ_CP063393:3690510-3691064 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT178124 NZ_CP083862:32025-32444 [Enterobacter kobei]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA