Detailed information of TA system TA178114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5336922..5337562
• Replicon: chromosome
• Accession: NZ_CP063391
• Organism: Pseudomonas aeruginosa strain ST167_d68blood2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IPV43_RS24720
• Protein ID: WP_003134109.1
• Coordinates: 5336922..5337332 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV43_RS24725
• Protein ID: WP_031634724.1
• Coordinates: 5337332..5337562 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV43_RS24680	5332448..5333245	+	798	WP_235597565.1	hypothetical protein	-
IPV43_RS24685 (IPV43_24685)	5333402..5334130	+	729	WP_004352842.1	TIGR03761 family integrating conjugative element protein	-
IPV43_RS24690 (IPV43_24690)	5334136..5334684	+	549	WP_004352841.1	DUF3158 family protein	-
IPV43_RS24695 (IPV43_24695)	5334731..5335570	+	840	WP_003105750.1	Rha family transcriptional regulator	-
IPV43_RS24700 (IPV43_24700)	5335600..5336088	+	489	WP_004352840.1	single-stranded DNA-binding protein	-
IPV43_RS24705 (IPV43_24705)	5336244..5336411	+	168	WP_128729268.1	CrpP family ICE-associated protein	-
IPV43_RS24710 (IPV43_24710)	5336477..5336695	-	219	WP_023083218.1	hypothetical protein	-
IPV43_RS24715 (IPV43_24715)	5336709..5336906	-	198	WP_023083219.1	hypothetical protein	-
IPV43_RS24720 (IPV43_24720)	5336922..5337332	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IPV43_RS24725 (IPV43_24725)	5337332..5337562	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IPV43_RS24730 (IPV43_24730)	5337818..5339737	+	1920	WP_004352838.1	type I DNA topoisomerase	-
IPV43_RS24735 (IPV43_24735)	5340045..5340254	+	210	WP_003105733.1	cold-shock protein	-
IPV43_RS24740 (IPV43_24740)	5340475..5342364	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5318209..5407409	89200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T178114 WP_003134109.1 NZ_CP063391:c5337332-5336922 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T178114 NZ_CP083857:31791-32030 [Enterobacter kobei]
CTGGGGCGAACCATCGAAAAGAGCCATTGCCCGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAA
GTATCTCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAA
AGTTTTATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT178114 WP_031634724.1 NZ_CP063391:c5337562-5337332 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT178114 NZ_CP083857:32027-32446 [Enterobacter kobei]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA