Detailed information of TA system TA178104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 142837..143342
• Replicon: chromosome
• Accession: NZ_CP063390
• Organism: Pseudomonas aeruginosa strain ST1076_d97burn1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: IPV44_RS00655
• Protein ID: WP_003083773.1
• Coordinates: 142837..143118 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: IPV44_RS00660
• Protein ID: WP_003083775.1
• Coordinates: 143115..143342 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV44_RS00630 (IPV44_00625)	138087..139436	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
IPV44_RS00635 (IPV44_00630)	139485..140171	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
IPV44_RS00640 (IPV44_00635)	140272..141006	+	735	WP_003110658.1	GntR family transcriptional regulator	-
IPV44_RS00645 (IPV44_00640)	141186..141596	+	411	WP_003110659.1	aegerolysin family protein	-
IPV44_RS00650 (IPV44_00645)	141628..142536	-	909	WP_003083769.1	LysR family transcriptional regulator	-
IPV44_RS00655 (IPV44_00650)	142837..143118	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
IPV44_RS00660 (IPV44_00655)	143115..143342	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
IPV44_RS00665 (IPV44_00660)	143518..144138	-	621	WP_003101226.1	hypothetical protein	-
IPV44_RS00670 (IPV44_00665)	144239..144739	+	501	WP_003101228.1	LEA type 2 family protein	-
IPV44_RS00675 (IPV44_00670)	144812..145153	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
IPV44_RS00680 (IPV44_00675)	145235..146662	-	1428	WP_003083784.1	GABA permease	-
IPV44_RS00685 (IPV44_00680)	146831..148324	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T178104 WP_003083773.1 NZ_CP063390:c143118-142837 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T178104 NZ_CP083853:507761-508045 [Enterobacter roggenkampii]
ATGACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGGATACGGTCAAAAACCAGTTTAA
GAAGAAACTTGCCGGGGTATTGGCAAATCCCCGGGTCGAATCGGCCCGCCTGCATAATCTGCCTGATTGCTACAAAATCA
AACTCAAGGCGTCTGGCTATCGCCTGGTTTATCAGGTTCAGGACAGCGTTATAACGGTATTTGTTATCGCCGTTGGGAAA
AGAGAGAAATCAGCCGCTTATCACGACGCAAATAAACGGCTTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT178104 WP_003083775.1 NZ_CP063390:c143342-143115 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT178104 NZ_CP083853:507529-507771 [Enterobacter roggenkampii]
ATGGCCACGCTAAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATTACCCC
AACGGAAGCGGTGCGATTGCTGTTTCAGTACGTTGCTGAAACGGGGCGCATGCCGGTGAAAACGGTCACCATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATAAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9