Detailed information of TA system TA178101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3615243..3616463
• Replicon: chromosome
• Accession: NZ_CP063389
• Organism: Pseudomonas aeruginosa strain ST1076_d97burn2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: IPV45_RS16610
• Protein ID: WP_003110488.1
• Coordinates: 3615885..3616463 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV45_RS16605
• Protein ID: WP_003132728.1
• Coordinates: 3615243..3615797 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV45_RS16580 (IPV45_16575)	3610811..3611362	+	552	WP_031685890.1	XRE family transcriptional regulator	-
IPV45_RS16585 (IPV45_16580)	3611359..3611757	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IPV45_RS16590 (IPV45_16585)	3611770..3612093	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IPV45_RS16595 (IPV45_16590)	3612424..3612885	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IPV45_RS16600 (IPV45_16595)	3612890..3615085	+	2196	WP_033957494.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IPV45_RS16605 (IPV45_16600)	3615243..3615797	+	555	WP_003132728.1	helix-turn-helix transcriptional regulator	Antitoxin
IPV45_RS16610 (IPV45_16605)	3615885..3616463	+	579	WP_003110488.1	HD domain-containing protein	Toxin
IPV45_RS16615 (IPV45_16610)	3616573..3617760	-	1188	WP_003110489.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IPV45_RS16620 (IPV45_16615)	3617750..3619921	-	2172	WP_033957491.1	type I secretion system permease/ATPase	-
IPV45_RS16625 (IPV45_16620)	3619911..3621188	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T178101 WP_003110488.1 NZ_CP063389:3615885-3616463 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T178101 NZ_CP083853:c80830-80462 [Enterobacter roggenkampii]
GTGGACGGTTCGGTTCGGTTCGGTTCGGTTCGGTTCGGAAAGGTATTCGAGCAGTGGTTGTTTGAACAAGAAGAAGGATT
ACAGGATAAGGTACTGGCAGACCTGCTAAATCTGCAACACTATGGGCCACGTCTTCCTCGTCCCTATGCAGATACGGTTA
AAGGCTCACGGTATAAGCATATGAAAGAATTACGTATTCAATATGCTGGTCGTCCTGTTCGTGCTTTCTTCGCATTTGAT
CCTGTACGTCAGGCCATTGTGCTTTGCGCGGGCGATAAGAGTAACGACAAAACCTTTTATGAAAAAATGATTCGCATCGC
AGATGCCGAATTTTCCCTTCATCTGACAAGCCAAGAGGCAGCCAAGTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT178101 WP_003132728.1 NZ_CP063389:3615243-3615797 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT178101 NZ_CP083853:c80465-80166 [Enterobacter roggenkampii]
GTGAAAACCTTAAATGAAGTGATTGCGTCACGTTCACCGGAAAGCCAACAACGTATTAAAGAAATGGCAGAGGAAATGAT
CCTCGAAACGGGTCTGCAACTTTTACGCGAAGAGCTCATGCTTTCGCAAAAAAAAGTTGCTGACCTGATGGGGGTGAGCC
AGCCCGCAGTAACTCAGATAGAGCAACGTGGAAATGAACTAAAAATTGCCACGCTTAAGCGTTACATCGAGGCAATGGGT
GGGAAACTCTGTATCGACGTTGAACTTCCCACAGGAAAACGCGTTGCATTTCATATCTAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4