Detailed information of TA system TA178095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3615232..3616452
• Replicon: chromosome
• Accession: NZ_CP063388
• Organism: Pseudomonas aeruginosa strain ST1076_d100blood1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: IPV46_RS16610
• Protein ID: WP_003110488.1
• Coordinates: 3615874..3616452 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV46_RS16605
• Protein ID: WP_003132728.1
• Coordinates: 3615232..3615786 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV46_RS16580 (IPV46_16575)	3610800..3611351	+	552	WP_031685890.1	XRE family transcriptional regulator	-
IPV46_RS16585 (IPV46_16580)	3611348..3611746	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IPV46_RS16590 (IPV46_16585)	3611759..3612082	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IPV46_RS16595 (IPV46_16590)	3612413..3612874	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IPV46_RS16600 (IPV46_16595)	3612879..3615074	+	2196	WP_033957494.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IPV46_RS16605 (IPV46_16600)	3615232..3615786	+	555	WP_003132728.1	helix-turn-helix transcriptional regulator	Antitoxin
IPV46_RS16610 (IPV46_16605)	3615874..3616452	+	579	WP_003110488.1	HD domain-containing protein	Toxin
IPV46_RS16615 (IPV46_16610)	3616562..3617749	-	1188	WP_003110489.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IPV46_RS16620 (IPV46_16615)	3617739..3619910	-	2172	WP_033957491.1	type I secretion system permease/ATPase	-
IPV46_RS16625 (IPV46_16620)	3619900..3621177	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T178095 WP_003110488.1 NZ_CP063388:3615874-3616452 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T178095 NZ_CP083849:c3155075-3154761 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT178095 WP_003132728.1 NZ_CP063388:3615232-3615786 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT178095 NZ_CP083849:c3154761-3154345 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGTCCTTTGCTGGATATTGTGTGCG
CCAGAATTAGCCATTACGAAGCTAATGGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4