Detailed information of TA system TA178089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3615242..3616462
• Replicon: chromosome
• Accession: NZ_CP063387
• Organism: Pseudomonas aeruginosa strain ST1076_d100blood2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: IPV47_RS16610
• Protein ID: WP_003110488.1
• Coordinates: 3615884..3616462 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV47_RS16605
• Protein ID: WP_003132728.1
• Coordinates: 3615242..3615796 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV47_RS16580 (IPV47_16575)	3610810..3611361	+	552	WP_031685890.1	XRE family transcriptional regulator	-
IPV47_RS16585 (IPV47_16580)	3611358..3611756	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IPV47_RS16590 (IPV47_16585)	3611769..3612092	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IPV47_RS16595 (IPV47_16590)	3612423..3612884	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IPV47_RS16600 (IPV47_16595)	3612889..3615084	+	2196	WP_033957494.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IPV47_RS16605 (IPV47_16600)	3615242..3615796	+	555	WP_003132728.1	helix-turn-helix transcriptional regulator	Antitoxin
IPV47_RS16610 (IPV47_16605)	3615884..3616462	+	579	WP_003110488.1	HD domain-containing protein	Toxin
IPV47_RS16615 (IPV47_16610)	3616572..3617759	-	1188	WP_003110489.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IPV47_RS16620 (IPV47_16615)	3617749..3619920	-	2172	WP_033957491.1	type I secretion system permease/ATPase	-
IPV47_RS16625 (IPV47_16620)	3619910..3621187	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T178089 WP_003110488.1 NZ_CP063387:3615884-3616462 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T178089 NZ_CP083842:c4667418-4667047 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATTGATCTATTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGCCTGATCACATGGATGGAAGTCATGGTGGGTGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT178089 WP_003132728.1 NZ_CP063387:3615242-3615796 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT178089 NZ_CP083842:c4667663-4667421 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4