Detailed information of TA system TA178083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3607439..3608659
• Replicon: chromosome
• Accession: NZ_CP063386
• Organism: Pseudomonas aeruginosa strain ST1076_d118limb1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: IPV48_RS16590
• Protein ID: WP_003110488.1
• Coordinates: 3608081..3608659 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IPV48_RS16585
• Protein ID: WP_003132728.1
• Coordinates: 3607439..3607993 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV48_RS16560 (IPV48_16550)	3603007..3603558	+	552	WP_031685890.1	XRE family transcriptional regulator	-
IPV48_RS16565 (IPV48_16555)	3603555..3603953	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
IPV48_RS16570 (IPV48_16560)	3603966..3604289	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
IPV48_RS16575 (IPV48_16565)	3604620..3605081	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
IPV48_RS16580 (IPV48_16570)	3605086..3607281	+	2196	WP_033957494.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
IPV48_RS16585 (IPV48_16575)	3607439..3607993	+	555	WP_003132728.1	helix-turn-helix transcriptional regulator	Antitoxin
IPV48_RS16590 (IPV48_16580)	3608081..3608659	+	579	WP_003110488.1	HD domain-containing protein	Toxin
IPV48_RS16595 (IPV48_16585)	3608769..3609956	-	1188	WP_003110489.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IPV48_RS16600 (IPV48_16590)	3609946..3612117	-	2172	WP_033957491.1	type I secretion system permease/ATPase	-
IPV48_RS16605 (IPV48_16595)	3612107..3613384	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T178083 WP_003110488.1 NZ_CP063386:3608081-3608659 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T178083 NZ_CP083842:c1020451-1020233 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTCGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAGCTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20470.63 Da
• Isoelectric Point: 8.1590

>AT178083 WP_003132728.1 NZ_CP063386:3607439-3607993 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT178083 NZ_CP083842:c1020853-1020479 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4