Detailed information of TA system TA178081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1613816..1615511
• Replicon: chromosome
• Accession: NZ_CP063386
• Organism: Pseudomonas aeruginosa strain ST1076_d118limb1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: IPV48_RS07515
• Protein ID: WP_003112901.1
• Coordinates: 1613816..1614685 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: IPV48_RS07520
• Protein ID: WP_003112902.1
• Coordinates: 1614678..1615511 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPV48_RS07490 (IPV48_07480)	1609824..1610675	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
IPV48_RS07495 (IPV48_07485)	1610716..1611723	+	1008	WP_023097315.1	ABC transporter substrate-binding protein	-
IPV48_RS07500 (IPV48_07490)	1611720..1612496	+	777	WP_003112898.1	ABC transporter permease	-
IPV48_RS07505 (IPV48_07495)	1612502..1613263	+	762	WP_003112899.1	SDR family oxidoreductase	-
IPV48_RS07510 (IPV48_07500)	1613277..1613807	+	531	WP_031685488.1	cupin domain-containing protein	-
IPV48_RS07515 (IPV48_07505)	1613816..1614685	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
IPV48_RS07520 (IPV48_07510)	1614678..1615511	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
IPV48_RS07525 (IPV48_07515)	1615498..1616295	+	798	WP_023111480.1	SDR family oxidoreductase	-
IPV48_RS07530 (IPV48_07520)	1616288..1617970	+	1683	WP_031685491.1	thiamine pyrophosphate-binding protein	-
IPV48_RS07535 (IPV48_07525)	1617981..1618784	+	804	WP_003130116.1	aspartate dehydrogenase	-
IPV48_RS07540 (IPV48_07530)	1618796..1620280	+	1485	WP_031685492.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T178081 WP_003112901.1 NZ_CP063386:1613816-1614685 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T178081 NZ_CP083836:2481-2771 [Enterobacter asburiae]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAATCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT178081 WP_003112902.1 NZ_CP063386:1614678-1615511 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT178081 NZ_CP083836:2237-2491 [Enterobacter asburiae]
ATGAGCACAATTCAAATCCGCGTTGATGACGAACTGAAAAAAGACGCCTATCAGGCCTTTGAAAAACTGAATCTGTCGCC
TTCAGACGCACTTCGTCTGTTCCTGCGGTATGTCGCAGAGAACGAAAAACTCCCCTTTGCTGAAGTCTCAGTGATGGTGA
GCGAAGATGATGAAGACGCTGATATTCTGGCAGTGGTTCGGGAAAGACTGAAAAATCCGGCAAGACGGATCAGGGTTAAC
GTTGATGACCTTTAA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1