Detailed information of TA system TA178072

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4396418..4397078
• Replicon: chromosome
• Accession: NZ_CP063380
• Organism: Pectobacterium versatile strain ECC15

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: IMY97_RS19970
• Protein ID: WP_103861404.1
• Coordinates: 4396418..4396831 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1V2R9A9
• Locus tag: IMY97_RS19975
• Protein ID: WP_039355397.1
• Coordinates: 4396812..4397078 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY97_RS19945 (IMY97_20105)	4392196..4393524	+	1329	WP_180790548.1	MFS transporter	-
IMY97_RS19950 (IMY97_20110)	4393749..4394135	+	387	WP_180790547.1	thioesterase family protein	-
IMY97_RS19955 (IMY97_20115)	4394478..4394996	+	519	WP_044204035.1	flavodoxin FldB	-
IMY97_RS19960 (IMY97_20120)	4395025..4395348	+	324	WP_103861402.1	hypothetical protein	-
IMY97_RS19965 (IMY97_20125)	4395414..4396349	-	936	WP_103861403.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
IMY97_RS19970 (IMY97_20130)	4396418..4396831	-	414	WP_103861404.1	protein YgfX	Toxin
IMY97_RS19975 (IMY97_20135)	4396812..4397078	-	267	WP_039355397.1	FAD assembly factor SdhE	Antitoxin
IMY97_RS19980 (IMY97_20140)	4397314..4398315	+	1002	WP_103861405.1	tRNA-modifying protein YgfZ	-
IMY97_RS19985 (IMY97_20145)	4398507..4399154	-	648	WP_103183036.1	hemolysin III family protein	-
IMY97_RS19990 (IMY97_20150)	4399433..4400041	+	609	WP_095701613.1	HD domain-containing protein	-
IMY97_RS19995 (IMY97_20155)	4400096..4401100	+	1005	WP_188246691.1	substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16359.31 Da
• Isoelectric Point: 10.7911

>T178072 WP_103861404.1 NZ_CP063380:c4396831-4396418 [Pectobacterium versatile]
VAQWRCDLRVSWRMQLLSLVVHGLLVLFILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGVLLSLQAVNAKDKQQLWLASDSMGDDEWRHLRQLLLQQKHWAR

Nucleotide

• Length: 414 bp

>T178072 NZ_CP083834:c3023866-3023513 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGAACGCGCCAGCGTACTTGC
AGCACTGCTGGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGAATAGATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10506.99 Da
• Isoelectric Point: 6.2223

>AT178072 WP_039355397.1 NZ_CP063380:c4397078-4396812 [Pectobacterium versatile]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPKDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT178072 NZ_CP083834:c3023508-3023206 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCTGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1V2R9A9