Detailed information of TA system TA178069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4143726..4144510
• Replicon: chromosome
• Accession: NZ_CP063380
• Organism: Pectobacterium versatile strain ECC15

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: IMY97_RS18900
• Protein ID: WP_116586074.1
• Coordinates: 4143726..4144220 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A855MHZ3
• Locus tag: IMY97_RS18905
• Protein ID: WP_010301483.1
• Coordinates: 4144217..4144510 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY97_RS18880 (IMY97_23360)	4139552..4141441	-	1890	WP_130640477.1	N-6 DNA methylase	-
IMY97_RS18885 (IMY97_19065)	4141694..4142818	-	1125	WP_038918036.1	site-specific integrase	-
IMY97_RS18890 (IMY97_19070)	4142935..4143159	-	225	WP_161452943.1	DUF1778 domain-containing protein	-
IMY97_RS18895 (IMY97_19075)	4143494..4143664	-	171	Protein_3706	helix-turn-helix domain-containing protein	-
IMY97_RS18900 (IMY97_19080)	4143726..4144220	-	495	WP_116586074.1	GNAT family N-acetyltransferase	Toxin
IMY97_RS18905 (IMY97_19085)	4144217..4144510	-	294	WP_010301483.1	DUF1778 domain-containing protein	Antitoxin
IMY97_RS18910 (IMY97_19090)	4144717..4145217	-	501	WP_103861241.1	serine acetyltransferase	-
IMY97_RS18915 (IMY97_19095)	4145244..4146638	-	1395	WP_103972346.1	DUF4832 domain-containing protein	-
IMY97_RS18920 (IMY97_19100)	4146677..4147084	-	408	WP_039490298.1	hypothetical protein	-
IMY97_RS18925 (IMY97_19105)	4147087..4148199	-	1113	WP_039507741.1	glycosyltransferase family 4 protein	-
IMY97_RS18930 (IMY97_19110)	4148201..4149412	-	1212	WP_103861689.1	glycosyltransferase family 4 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17571.33 Da
• Isoelectric Point: 7.9094

>T178069 WP_116586074.1 NZ_CP063380:c4144220-4143726 [Pectobacterium versatile]
MISAPEPLHAEHVFSSFGCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARNFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T178069 NZ_CP083834:c1055910-1055692 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10930.52 Da
• Isoelectric Point: 10.1968

>AT178069 WP_010301483.1 NZ_CP063380:c4144510-4144217 [Pectobacterium versatile]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQTPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT178069 NZ_CP083834:c1056312-1055938 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A855MHZ3