Detailed information of TA system TA178063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 432927..433537
• Replicon: chromosome
• Accession: NZ_CP063380
• Organism: Pectobacterium versatile strain ECC15

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: IMY97_RS01980
• Protein ID: WP_242934920.1
• Coordinates: 432927..433319 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: C6DIE5
• Locus tag: IMY97_RS01985
• Protein ID: WP_010300679.1
• Coordinates: 433316..433537 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY97_RS01945 (IMY97_01910)	428229..429410	+	1182	WP_103862790.1	AI-2E family transporter	-
IMY97_RS01950 (IMY97_01915)	429477..429839	-	363	WP_103862789.1	DUF596 domain-containing protein	-
IMY97_RS01955 (IMY97_01920)	429875..430261	-	387	WP_180744329.1	hypothetical protein	-
IMY97_RS01960 (IMY97_01925)	430375..430920	+	546	WP_107332125.1	hypothetical protein	-
IMY97_RS01965 (IMY97_01930)	430963..431520	-	558	WP_105880054.1	hypothetical protein	-
IMY97_RS01970 (IMY97_22455)	431875..432111	-	237	WP_242934919.1	hypothetical protein	-
IMY97_RS01975 (IMY97_22460)	432146..432586	-	441	WP_198673503.1	class I SAM-dependent methyltransferase	-
IMY97_RS01980 (IMY97_01940)	432927..433319	-	393	WP_242934920.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IMY97_RS01985 (IMY97_01945)	433316..433537	-	222	WP_010300679.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IMY97_RS01990 (IMY97_01950)	433778..434989	-	1212	Protein_389	IS3 family transposase	-
IMY97_RS01995 (IMY97_01955)	435847..436371	+	525	WP_103862785.1	hypothetical protein	-
IMY97_RS02000 (IMY97_01960)	436400..437287	+	888	WP_180790295.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
IMY97_RS02005 (IMY97_01965)	437393..438256	-	864	WP_095993164.1	YicC/YloC family endoribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rfaD	337966..510621	172655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (3-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14719.67 Da
• Isoelectric Point: 6.6442

>T178063 WP_242934920.1 NZ_CP063380:c433319-432927 [Pectobacterium versatile]
MNWVSAQEVIAFHDRILRHLPGVAGMPDPGRAEAIIYRVQNRTHYEGITDVFELAAIYWVAVARGHIFNDGNKRTAFFVT
MTFLYRNGVLIRDNDNTLENLTVEAATGPKNVDQLAQHLRNLADEISPTR

Nucleotide

• Length: 393 bp

>T178063 NZ_CP083832:c46358-45948 [Enterobacter asburiae]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTGGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.01 Da
• Isoelectric Point: 5.0359

>AT178063 WP_010300679.1 NZ_CP063380:c433537-433316 [Pectobacterium versatile]
MRTYTSTQARANISEVLDAATHGEPVEITRRDGSSAVVISKAEFEAYQNAKLDAEFDLIMQRHGHTIEALTDR

Nucleotide

• Length: 222 bp

>AT178063 NZ_CP083832:c46585-46355 [Enterobacter asburiae]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A221TFG2