Detailed information of TA system TA178059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 92619..93274
• Replicon: chromosome
• Accession: NZ_CP063380
• Organism: Pectobacterium versatile strain ECC15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IMY97_RS00390
• Protein ID: WP_242934906.1
• Coordinates: 92978..93274 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A221T3Z5
• Locus tag: IMY97_RS00385
• Protein ID: WP_010308714.1
• Coordinates: 92619..92981 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMY97_RS00380 (IMY97_00325)	88616..92359	-	3744	WP_188246897.1	DUF4132 domain-containing protein	-
IMY97_RS00385 (IMY97_00330)	92619..92981	-	363	WP_010308714.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IMY97_RS00390 (IMY97_00335)	92978..93274	-	297	WP_242934906.1	type II toxin-antitoxin system HicA family toxin	Toxin
IMY97_RS00395 (IMY97_00340)	93486..94010	-	525	WP_188246896.1	cell-envelope stress modulator CpxP	-
IMY97_RS00400 (IMY97_00345)	94164..94862	+	699	WP_010308725.1	envelope stress response regulator transcription factor CpxR	-
IMY97_RS00405 (IMY97_00350)	94859..96229	+	1371	WP_103862317.1	envelope stress sensor histidine kinase CpxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (37-92)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10883.59 Da
• Isoelectric Point: 11.3874

>T178059 WP_242934906.1 NZ_CP063380:c93274-92978 [Pectobacterium versatile]
MTSWNRSCRGKIASLRKKQRETLSHIFKTPILSGVKWSSIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTD
KGALVSLREWLESIGVKP

Nucleotide

• Length: 297 bp

>T178059 NZ_CP083830:c4307422-4307138 [Enterobacter asburiae]
ATGACTTATGAACTGGCATTTGACCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGCAGACTGTAAAAGACCAGTTTAA
GAAGAAACTTGCGGACGTGCTGGTGAACCCCAGGGTCGAATCGGCCCGCCTGCATGGTCTGCCGGATTGCTACAAAATAA
AACTCAGAACGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAACGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
CGAGAGAAATCAGCCGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13495.29 Da
• Isoelectric Point: 4.6225

>AT178059 WP_010308714.1 NZ_CP063380:c92981-92619 [Pectobacterium versatile]
MTKALNTPNTMVVSGQPAIISYVPEIGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERHISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT178059 NZ_CP083830:c4307654-4307412 [Enterobacter asburiae]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACGGAAGCCGTGCGATTACTGTTTCAGTACGTCGCGGAGACGGGACGTATGCCGGTGAAAACGGTCACGATTAGCGACA
GTGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCGAATCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A221T3Z5