Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42071..42596 Replicon plasmid p108.7k
Accession NZ_CP063371
Organism Escherichia coli strain 1409

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FOI11_RS24090 Protein ID WP_001159871.1
Coordinates 42291..42596 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag FOI11_RS24085 Protein ID WP_000813639.1
Coordinates 42071..42289 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOI11_RS24060 37898..39778 + 1881 WP_001283356.1 colicin -
FOI11_RS24065 39796..40143 - 348 WP_000762580.1 hypothetical protein -
FOI11_RS24070 40262..40609 + 348 WP_000142424.1 hypothetical protein -
FOI11_RS24075 40627..41217 - 591 WP_000194555.1 hypothetical protein -
FOI11_RS24080 41214..41474 - 261 WP_000343102.1 hypothetical protein -
FOI11_RS24085 42071..42289 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FOI11_RS24090 42291..42596 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FOI11_RS24095 42597..43403 + 807 WP_033904598.1 site-specific integrase -
FOI11_RS24100 43583..44227 + 645 WP_001144036.1 ParA family protein -
FOI11_RS24105 44314..44622 + 309 WP_000030199.1 hypothetical protein -
FOI11_RS24110 45036..46016 + 981 WP_000688514.1 plasmid segregation protein ParM -
FOI11_RS24115 46009..46425 + 417 WP_001278818.1 plasmid partitioning/stability family protein -
FOI11_RS24120 46427..46600 - 174 Protein_53 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul1 / qacE / aac(3)-VIa / ant(3'')-Ia / dfrA1 - 1..108699 108699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T178053 WP_001159871.1 NZ_CP063371:42291-42596 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T178053 NZ_CP083830:863429-863839 [Enterobacter asburiae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTACGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT178053 WP_000813639.1 NZ_CP063371:42071-42289 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT178053 NZ_CP083830:863182-863448 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTTGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References