Detailed information of TA system TA178047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 41381..41928
• Replicon: plasmid p113.6k
• Accession: NZ_CP063370
• Organism: Escherichia coli strain 1409

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: FOI11_RS23400
• Protein ID: WP_000969996.1
• Coordinates: 41381..41662 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A3A1Q2G3
• Locus tag: FOI11_RS23405
• Protein ID: WP_042021804.1
• Coordinates: 41659..41928 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOI11_RS23365	36484..37410	+	927	WP_080077783.1	hypothetical protein	-
FOI11_RS23370	37460..37714	+	255	WP_059291112.1	hypothetical protein	-
FOI11_RS23375	38212..38556	+	345	WP_087601730.1	abortive infection protein	-
FOI11_RS23380	38730..39731	-	1002	WP_000774874.1	DUF4238 domain-containing protein	-
FOI11_RS23385	40082..40339	+	258	WP_000371888.1	hypothetical protein	-
FOI11_RS23390	40339..40929	+	591	WP_000194553.1	hypothetical protein	-
FOI11_RS23395	40949..41296	-	348	WP_024148462.1	hypothetical protein	-
FOI11_RS23400	41381..41662	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOI11_RS23405	41659..41928	-	270	WP_042021804.1	hypothetical protein	Antitoxin
FOI11_RS23410	41983..42141	-	159	WP_000829588.1	hypothetical protein	-
FOI11_RS23415	42844..43701	-	858	WP_001697864.1	incFII family plasmid replication initiator RepA	-
FOI11_RS23420	43694..43768	-	75	WP_001375168.1	RepA leader peptide Tap	-
FOI11_RS23425	44004..44261	-	258	WP_000083826.1	replication regulatory protein RepA	-
FOI11_RS23430	44501..45091	-	591	WP_000766805.1	DUF2726 domain-containing protein	-
FOI11_RS23435	45129..45338	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
FOI11_RS23440	45384..45845	-	462	WP_001233838.1	thermonuclease family protein	-
FOI11_RS23445	46090..46302	-	213	WP_001309245.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN	1..113611	113611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T178047 WP_000969996.1 NZ_CP063370:c41662-41381 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T178047 NZ_CP083828:c1085428-1085210 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10122.48 Da
• Isoelectric Point: 10.2879

>AT178047 WP_042021804.1 NZ_CP063370:c41928-41659 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT178047 NZ_CP083828:c1085830-1085456 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A3A1Q2G3