Detailed information of TA system TA178046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4662960..4663543
• Replicon: chromosome
• Accession: NZ_CP063369
• Organism: Escherichia coli strain 1409

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: FOI11_RS22270
• Protein ID: WP_000254738.1
• Coordinates: 4663208..4663543 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: FOI11_RS22265
• Protein ID: WP_000581937.1
• Coordinates: 4662960..4663208 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOI11_RS22255	4659299..4660600	+	1302	WP_001556187.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
FOI11_RS22260	4660648..4662882	+	2235	WP_032176554.1	GTP diphosphokinase	-
FOI11_RS22265	4662960..4663208	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FOI11_RS22270	4663208..4663543	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
FOI11_RS22275	4663614..4664405	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
FOI11_RS22280	4664633..4666270	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
FOI11_RS22285	4666358..4667656	+	1299	WP_000036723.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T178046 WP_000254738.1 NZ_CP063369:4663208-4663543 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T178046 NZ_CP083821:c4670074-4669763 [Enterobacter cloacae subsp. cloacae]
ATGCTATTCATTGAAACGGATATCTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTCCAGGT
GTTCCTGGCAACACAACCTGATTATGGCGATCTGATCCAGAATACCGGGGGGCTGCGAAAGATTCGTTGGCTTTCAGGAG
GCAGGGGAAAGCGCGGTGGAGTAAGGGTTATTTATTTTCATCGTACCCACGAGTTTGAAATCAGATTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAGAGAAAAAGCCATTCTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT178046 WP_000581937.1 NZ_CP063369:4662960-4663208 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT178046 NZ_CP083821:c4669762-4669472 [Enterobacter cloacae subsp. cloacae]
ATGGATAAGGCGCTGTTTGAGCGACTAACACAAAGCATAGCTCAGATGGATGACATTGTCGGGGGGAAACGCGAGCCGTC
GCGCACCTTTCAAATCGATGCGATGAAAATCAAAGAAATTCGTCAGGCGTCTGGGTTGTCCCAAAGTAAATTCGCGAACT
TAATCTCAGTCAGCGTTGATACGTTGCGTAACTGGGAACAAGGACGACGTTCGCCTACCGGTCCCGCAAAGGCATTGTTG
CGTGCAATTGCGAATGATCCACAGCACGTTATTTCGGCTTTATCAGATTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4