Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4525017..4525671 Replicon chromosome
Accession NZ_CP063369
Organism Escherichia coli strain 1409

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FOI11_RS21640 Protein ID WP_000244777.1
Coordinates 4525264..4525671 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FOI11_RS21635 Protein ID WP_000354046.1
Coordinates 4525017..4525283 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOI11_RS21615 4520986..4522419 - 1434 WP_141095424.1 6-phospho-beta-glucosidase BglA -
FOI11_RS21620 4522464..4522775 + 312 WP_001514524.1 N(4)-acetylcytidine aminohydrolase -
FOI11_RS21625 4522939..4523598 + 660 WP_000250274.1 hemolysin III family protein -
FOI11_RS21630 4523794..4524774 - 981 WP_001514522.1 tRNA-modifying protein YgfZ -
FOI11_RS21635 4525017..4525283 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FOI11_RS21640 4525264..4525671 + 408 WP_000244777.1 toxin CptA Toxin
FOI11_RS21645 4525711..4526232 - 522 WP_001055874.1 flavodoxin FldB -
FOI11_RS21650 4526344..4527240 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FOI11_RS21655 4527265..4527975 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FOI11_RS21660 4527981..4529714 + 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T178045 WP_000244777.1 NZ_CP063369:4525264-4525671 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T178045 NZ_CP083821:c3893022-3892531 [Enterobacter cloacae subsp. cloacae]
ATGATCTCTGCCCCAGAGCCCCTTCACGCCGGTCATATTCTTACTCCATTTTGCTGTGGTGTGGATTCGATGGATAACTG
GCTGAAGCAGCGGGCGATGAAAAATCAGGTCAGTGGTGCATCCCGTACCTTTGTCTGTTGTGGCAACGATTCTAACGTTC
AGGCTTATTACTCGCTGGCATCCAGTGCGGTCATGACGAATACCGCACCCGGTCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCGGTTGTTGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTGCATGGGCAGGGCGTTGGTCGGGCACTGGTACGCGA
CGCTGGGCTGCGGGTGATCCAGGTGGCGGAGACCATCGGCATTCGCGGAATGCTGGTTCATGCGCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCAATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGATG
GCGAGTATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT178045 WP_000354046.1 NZ_CP063369:4525017-4525283 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT178045 NZ_CP083821:c3893312-3893019 [Enterobacter cloacae subsp. cloacae]
ATGCCAGCTGCAAACACTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAGGCCTGCCGAGCGAGATCTTATCGA
TCGGGCGGCGAAAGCGAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCAGCCCGCGCTGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTGGCTCGTCTGGATCAGGCTCCAGCACCTAAT
GCCACACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCGGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References