Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3107052..3107549 Replicon chromosome
Accession NZ_CP063369
Organism Escherichia coli strain 1409

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FOI11_RS14985 Protein ID WP_001173343.1
Coordinates 3107052..3107225 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FOI11_RS14990 Protein ID WP_000398619.1
Coordinates 3107253..3107549 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOI11_RS14975 3103722..3105179 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
FOI11_RS14980 3105416..3106933 + 1518 WP_001295074.1 lysine--tRNA ligase -
FOI11_RS14985 3107052..3107225 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FOI11_RS14990 3107253..3107549 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FOI11_RS14995 3107776..3108048 - 273 WP_000405651.1 N-acetyltransferase -
FOI11_RS15000 3108060..3108290 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
FOI11_RS15005 3108471..3110102 + 1632 WP_001216477.1 sensor histidine kinase -
FOI11_RS15010 3110099..3110818 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T178040 WP_001173343.1 NZ_CP063369:c3107225-3107052 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T178040 NZ_CP083821:c1167112-1166750 [Enterobacter cloacae subsp. cloacae]
ATGTGGAATGTCGAGACAACAGACAGATTTGATGAATGGTTCTTTGAACAAACAACAGCGTTAAAAGAAGATGTGCTGGC
AGCAATGCATATTCTTTCTGAGTTTGGTCCGCAACTTGGGCGGCCGTTTGTAGACACCGTGAAGGCTTCAGCCTTTGGGA
ACATGAAAGAGTTACGAATTCAGCACAGAGGAAATCCCATCAGGGCCTTTTTTGCTTTTGATCCTGACCGCAAAGCAATT
GTGCTCTGTGCCGGGGACAAAACCGGTTGCAACCAAAAACGGTTTTATAGAGAGATGATAGCGCTGGCTGACGTTGAGTA
TCACAGACATCTGGCGAATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT178040 WP_000398619.1 NZ_CP063369:c3107549-3107253 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT178040 NZ_CP083821:c1166762-1166463 [Enterobacter cloacae subsp. cloacae]
ATGGCAACGCTAAAAGAACTTATGGCACAACAGAGCGCGGAAAGTCAGGAGCGCATTGCGGCGAAAGTTGAAGTGATGCG
CAGAATTGTTGCGCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCCACGGTGGCGAAAATCGAACAGCCGGGAAACGACCCCCGACTTTCAACGCTCAAACGCTATGTGAGTGCGCTTGGA
GGGGAGCTAAGCATCGATGTGACGTTACCAAACGGGAAACGGATAGCGTTTGAGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References