Detailed information of TA system TA178020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezT/zeta-couple_hipB
• Location: 591775..592992
• Replicon: chromosome
• Accession: NZ_CP063367
• Organism: Staphylococcus delphini strain 2794-1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: IPU22_RS02495
• Protein ID: WP_212575147.1
• Coordinates: 592252..592992 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: IPU22_RS02490
• Protein ID: WP_096543209.1
• Coordinates: 591775..592251 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IPU22_RS02465 (IPU22_02465)	587320..587895	+	576	WP_212575145.1	nitrate reductase molybdenum cofactor assembly chaperone	-
IPU22_RS02470 (IPU22_02470)	587888..588565	+	678	WP_212575146.1	respiratory nitrate reductase subunit gamma	-
IPU22_RS02475 (IPU22_02475)	588581..589042	+	462	WP_096543211.1	nitrate respiration regulation accessory nitrate sensor NreA	-
IPU22_RS02480 (IPU22_02480)	589043..590077	+	1035	WP_096543210.1	sensor histidine kinase	-
IPU22_RS02485 (IPU22_02485)	590096..590746	+	651	WP_019167019.1	nitrate respiration regulation response regulator NreC	-
IPU22_RS02490 (IPU22_02490)	591775..592251	+	477	WP_096543209.1	helix-turn-helix domain-containing protein	Antitoxin
IPU22_RS02495 (IPU22_02495)	592252..592992	+	741	WP_212575147.1	zeta toxin family protein	Toxin
IPU22_RS02500 (IPU22_02500)	593179..593511	-	333	WP_212575148.1	hypothetical protein	-
IPU22_RS02505 (IPU22_02505)	593835..594998	+	1164	WP_212575149.1	nitrate/nitrite transporter	-
IPU22_RS02510 (IPU22_02510)	595128..596723	-	1596	WP_212575150.1	M20 family metallopeptidase	-
IPU22_RS02515 (IPU22_02515)	596907..597263	+	357	WP_096543203.1	DUF3139 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-213)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28560.87 Da
• Isoelectric Point: 9.3372

>T178020 WP_212575147.1 NZ_CP063367:592252-592992 [Staphylococcus delphini]
MGLIEYTKIDFQTALERNYRGLTRGKTPIKRPIAILLGGQSGAGKTTIHRIKQSHYNGNIIIIDGDTFRLQHPKFIDIQK
EYGKKSVEYTNEFAGRMVESLIELLSEQHYHLLIEGTLRTVDVPRRTAVSLKSKGYQVELSLIATKPYLSYLSTLLRYEQ
NYSISPKYARATPKKYHDNIVGNLVENLNILEKEKIFDRIQIFKRDKSCVYDSYKNGELMASEKLHELLYGVWDLKEKEM
LEYCEN

Nucleotide

• Length: 741 bp

>T178020 NZ_CP083815:1626712-1627212 [Enterobacter asburiae]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGTGTCCTGGGTTATT
ACACGTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGAATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17947.45 Da
• Isoelectric Point: 4.5010

>AT178020 WP_096543209.1 NZ_CP063367:591775-592251 [Staphylococcus delphini]
MIGQNIKSLRQSKALTQPEFANMIGISRNSLSRYENGTSSITIGLIDRICEKFDVSYLDIVGKDKLLTPLEEYQLSFKIE
TTKERGASLLAELFDFQNKQGITTDDDTNPWILMSDDLSELINTKIYLVENFNTLERYIGYIDGIEHMLEVAKGRVVA

Nucleotide

• Length: 477 bp

>AT178020 NZ_CP083815:1626415-1626684 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA