Detailed information of TA system TA177988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 72754..73355
• Replicon: plasmid pBS15R-A_1
• Accession: NZ_CP063350
• Organism: Escherichia coli strain BS15R-A

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: IN859_RS24005
• Protein ID: WP_001216045.1
• Coordinates: 72975..73355 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: IN859_RS24000
• Protein ID: WP_001190712.1
• Coordinates: 72754..72975 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN859_RS23965	68128..68691	+	564	WP_001097412.1	chlorite dismutase family protein	-
IN859_RS23970	68942..69745	-	804	Protein_73	IS66 family transposase	-
IN859_RS23975	69798..70502	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
IN859_RS23980	71112..71252	+	141	WP_015974277.1	hypothetical protein	-
IN859_RS23985	71288..71539	+	252	WP_001667237.1	DNA polymerase III subunit theta	-
IN859_RS23990	71546..72118	-	573	WP_058664396.1	hypothetical protein	-
IN859_RS23995	72292..72681	+	390	WP_000506730.1	DNA repair protein	-
IN859_RS24000	72754..72975	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IN859_RS24005	72975..73355	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IN859_RS24010	73360..73539	+	180	WP_000113019.1	hypothetical protein	-
IN859_RS24015	73567..74610	+	1044	WP_000648833.1	DUF968 domain-containing protein	-
IN859_RS24020	74699..75151	+	453	WP_001326849.1	late promoter-activating protein	-
IN859_RS24025	75237..76430	+	1194	WP_000219618.1	hypothetical protein	-
IN859_RS24030	76430..77914	+	1485	WP_000124159.1	hypothetical protein	-
IN859_RS24035	78136..78255	+	120	WP_071526433.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / qnrS1 / blaTEM-1B	-	1..121017	121017
	-	flank	IS/Tn	-	-	68942..69718	776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T177988 WP_001216045.1 NZ_CP063350:72975-73355 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T177988 NZ_CP083797:c138733-138251 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCGAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT177988 WP_001190712.1 NZ_CP063350:72754-72975 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT177988 NZ_CP083797:c138987-138721 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6