Detailed information of TA system TA177987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4669538..4670163
• Replicon: chromosome
• Accession: NZ_CP063349
• Organism: Escherichia coli strain BS15R-A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IN859_RS22765
• Protein ID: WP_000911330.1
• Coordinates: 4669538..4669936 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IN859_RS22770
• Protein ID: WP_000450524.1
• Coordinates: 4669936..4670163 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN859_RS22745	4665416..4665616	+	201	WP_000383836.1	YpfN family protein	-
IN859_RS22750	4665726..4666424	-	699	WP_203007832.1	esterase	-
IN859_RS22755	4666498..4668513	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
IN859_RS22760	4668528..4669391	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
IN859_RS22765	4669538..4669936	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IN859_RS22770	4669936..4670163	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IN859_RS22775	4670318..4671031	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IN859_RS22780	4671244..4672278	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
IN859_RS22785	4672295..4673173	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IN859_RS22790	4673319..4673891	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IN859_RS22795	4673891..4674361	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T177987 WP_000911330.1 NZ_CP063349:c4669936-4669538 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T177987 NZ_CP083797:118327-118743 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCTGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGCCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT177987 WP_000450524.1 NZ_CP063349:c4670163-4669936 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT177987 NZ_CP083797:118100-118330 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33