Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2465491..2466109 Replicon chromosome
Accession NZ_CP063349
Organism Escherichia coli strain BS15R-A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IN859_RS11815 Protein ID WP_001291435.1
Coordinates 2465491..2465709 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IN859_RS11820 Protein ID WP_000344800.1
Coordinates 2465735..2466109 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN859_RS11780 2460781..2461353 + 573 WP_000779826.1 lipoprotein -
IN859_RS11785 2461384..2461695 - 312 WP_000409911.1 MGMT family protein -
IN859_RS11795 2462074..2462427 + 354 WP_000878141.1 DUF1428 family protein -
IN859_RS11800 2462469..2464019 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IN859_RS11805 2464183..2464653 - 471 WP_000136192.1 YlaC family protein -
IN859_RS11810 2464769..2465320 - 552 WP_000102578.1 maltose O-acetyltransferase -
IN859_RS11815 2465491..2465709 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
IN859_RS11820 2465735..2466109 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IN859_RS11825 2466655..2469804 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
IN859_RS11830 2469827..2471020 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T177977 WP_001291435.1 NZ_CP063349:c2465709-2465491 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T177977 NZ_CP083796:357648-357995 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT177977 WP_000344800.1 NZ_CP063349:c2466109-2465735 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT177977 NZ_CP083796:357995-358294 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References