Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2431183..2432020 Replicon chromosome
Accession NZ_CP063349
Organism Escherichia coli strain BS15R-A

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag IN859_RS11645 Protein ID WP_000227784.1
Coordinates 2431183..2431725 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag IN859_RS11650 Protein ID WP_001297137.1
Coordinates 2431709..2432020 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN859_RS11625 2426722..2427633 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
IN859_RS11630 2427801..2428292 + 492 WP_001138904.1 nucleotide binding protein YajQ -
IN859_RS11635 2428420..2429784 - 1365 WP_001000978.1 MFS transporter -
IN859_RS11640 2430192..2431127 + 936 WP_001297127.1 sel1 repeat family protein -
IN859_RS11645 2431183..2431725 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
IN859_RS11650 2431709..2432020 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
IN859_RS11655 2432205..2433095 - 891 WP_000971336.1 heme o synthase -
IN859_RS11660 2433107..2433436 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
IN859_RS11665 2433436..2434050 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
IN859_RS11670 2434040..2436031 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
IN859_RS11675 2436053..2437000 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T177976 WP_000227784.1 NZ_CP063349:c2431725-2431183 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T177976 NZ_CP083796:331677-332045 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT177976 WP_001297137.1 NZ_CP063349:c2432020-2431709 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT177976 NZ_CP083796:331459-331680 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References