Detailed information of TA system TA177966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 108846..109447
• Replicon: plasmid pBS90R-A_2
• Accession: NZ_CP063343
• Organism: Escherichia coli strain BS90R-A

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: IN877_RS24175
• Protein ID: WP_001216034.1
• Coordinates: 109067..109447 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: IN877_RS24170
• Protein ID: WP_001190712.1
• Coordinates: 108846..109067 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN877_RS24145	104203..104556	-	354	Protein_115	hypothetical protein	-
IN877_RS24150	104572..105269	-	698	Protein_116	IS1 family transposase	-
IN877_RS24155	105325..105705	-	381	Protein_117	type I restriction endonuclease subunit R	-
IN877_RS24160	105827..107110	-	1284	WP_001617890.1	restriction endonuclease subunit S	-
IN877_RS24165	107107..108663	-	1557	WP_001617892.1	type I restriction-modification system subunit M	-
IN877_RS24170	108846..109067	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
IN877_RS24175	109067..109447	+	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
IN877_RS24180	109452..109631	+	180	WP_001513661.1	hypothetical protein	-
IN877_RS24185	109659..110018	+	360	WP_001513660.1	hypothetical protein	-
IN877_RS24190	109942..110355	+	414	Protein_124	DDE-type integrase/transposase/recombinase	-
IN877_RS24195	110305..110622	-	318	WP_001513659.1	hypothetical protein	-
IN877_RS24200	110850..111866	-	1017	WP_077253135.1	IS5-like element IS5 family transposase	-
IN877_RS24205	112074..113477	+	1404	WP_203115767.1	S-methylmethionine permease	-
IN877_RS24210	113464..114396	+	933	WP_000081352.1	homocysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / tet(M)	-	1..117739	117739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T177966 WP_001216034.1 NZ_CP063343:109067-109447 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T177966 NZ_CP083790:774721-775131 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT177966 WP_001190712.1 NZ_CP063343:108846-109067 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT177966 NZ_CP083790:774474-774740 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6