Detailed information of TA system TA177950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2485426..2486064
• Replicon: chromosome
• Accession: NZ_CP063341
• Organism: Escherichia coli strain BS90R-A

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: IN877_RS11940
• Protein ID: WP_000813794.1
• Coordinates: 2485888..2486064 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IN877_RS11935
• Protein ID: WP_001270286.1
• Coordinates: 2485426..2485842 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN877_RS11915	2480578..2481519	-	942	WP_001251313.1	ABC transporter permease	-
IN877_RS11920	2481520..2482533	-	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
IN877_RS11925	2482551..2483696	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
IN877_RS11930	2483941..2485347	-	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
IN877_RS11935	2485426..2485842	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
IN877_RS11940	2485888..2486064	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
IN877_RS11945	2486286..2486516	+	231	WP_000494244.1	YncJ family protein	-
IN877_RS11950	2486608..2488569	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
IN877_RS11955	2488642..2489178	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
IN877_RS11960	2489231..2490445	+	1215	WP_071609973.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2490485..2491633	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T177950 WP_000813794.1 NZ_CP063341:c2486064-2485888 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T177950 NZ_CP083784:362096-362443 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT177950 WP_001270286.1 NZ_CP063341:c2485842-2485426 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT177950 NZ_CP083784:362443-362742 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• PDB: 6HPB