Detailed information of TA system TA177939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1339656..1340281
• Replicon: chromosome
• Accession: NZ_CP063341
• Organism: Escherichia coli strain BS90R-A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IN877_RS06445
• Protein ID: WP_000911330.1
• Coordinates: 1339883..1340281 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: IN877_RS06440
• Protein ID: WP_000450524.1
• Coordinates: 1339656..1339883 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN877_RS06415	1335459..1335929	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
IN877_RS06420	1335929..1336501	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
IN877_RS06425	1336647..1337525	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
IN877_RS06430	1337542..1338576	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
IN877_RS06435	1338789..1339502	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
IN877_RS06440	1339656..1339883	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IN877_RS06445	1339883..1340281	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IN877_RS06450	1340428..1341291	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
IN877_RS06455	1341306..1343321	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
IN877_RS06460	1343395..1344093	+	699	WP_000679823.1	esterase	-
IN877_RS06465	1344203..1344403	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T177939 WP_000911330.1 NZ_CP063341:1339883-1340281 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T177939 NZ_CP083775:4193303-4193521 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT177939 WP_000450524.1 NZ_CP063341:1339656-1339883 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT177939 NZ_CP083775:4192902-4193276 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33