177937

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1011025..1011608 Replicon chromosome
Accession NZ_CP063341
Organism Escherichia coli strain BS90R-A

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag IN877_RS04860 Protein ID WP_000254745.1
Coordinates 1011273..1011608 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag IN877_RS04855 Protein ID WP_000581937.1
Coordinates 1011025..1011273 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN877_RS04845 1007364..1008665 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
IN877_RS04850 1008713..1010947 + 2235 WP_000226815.1 GTP pyrophosphokinase -
IN877_RS04855 1011025..1011273 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
IN877_RS04860 1011273..1011608 + 336 WP_000254745.1 endoribonuclease MazF Toxin
IN877_RS04865 1011679..1012470 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
IN877_RS04870 1012698..1014335 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
IN877_RS04875 1014423..1015721 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T177937 WP_000254745.1 NZ_CP063341:1011273-1011608 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T177937 NZ_CP083775:2014604-2014706 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT177937 WP_000581937.1 NZ_CP063341:1011025-1011273 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT177937 NZ_CP083775:c2014713-2014568 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References