177934

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 294975..295775 Replicon chromosome
Accession NZ_CP063341
Organism Escherichia coli strain BS90R-A

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag IN877_RS01335 Protein ID WP_000342449.1
Coordinates 295248..295775 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag IN877_RS01330 Protein ID WP_001277108.1
Coordinates 294975..295241 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN877_RS01310 290633..291301 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
IN877_RS01315 291294..292352 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
IN877_RS01320 292597..293451 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
IN877_RS01325 293722..294825 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
IN877_RS01330 294975..295241 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
IN877_RS01335 295248..295775 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
IN877_RS01340 295772..296155 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
IN877_RS01345 296579..297688 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
IN877_RS01350 297736..298662 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
IN877_RS01355 298659..299936 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
IN877_RS01360 299933..300700 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T177934 WP_000342449.1 NZ_CP063341:295248-295775 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T177934 NZ_CP083775:338994-339362 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTTATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT177934 WP_001277108.1 NZ_CP063341:294975-295241 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT177934 NZ_CP083775:338776-338997 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A829CN24

References