Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 36218..36861 | Replicon | plasmid pBS74R-D_5 |
| Accession | NZ_CP063337 | ||
| Organism | Escherichia coli strain BS74R-D | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | A0A6H2GRP8 |
| Locus tag | IN878_RS25055 | Protein ID | WP_023292131.1 |
| Coordinates | 36218..36634 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | A0A6B7Q5S4 |
| Locus tag | IN878_RS25060 | Protein ID | WP_021312476.1 |
| Coordinates | 36631..36861 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IN878_RS25010 | 31568..31876 | + | 309 | Protein_34 | DDE-type integrase/transposase/recombinase | - |
| IN878_RS25015 | 31906..32115 | - | 210 | WP_094308932.1 | hypothetical protein | - |
| IN878_RS25020 | 32164..32367 | - | 204 | WP_004150739.1 | hemolysin expression modulator Hha | - |
| IN878_RS25025 | 32428..32922 | - | 495 | WP_094313750.1 | DNA-binding protein | - |
| IN878_RS25030 | 32953..33525 | - | 573 | WP_094309628.1 | hypothetical protein | - |
| IN878_RS25035 | 33530..33778 | - | 249 | WP_065801593.1 | hypothetical protein | - |
| IN878_RS25040 | 34057..35063 | - | 1007 | Protein_40 | IS110 family transposase | - |
| IN878_RS25045 | 35620..35874 | - | 255 | WP_095153353.1 | hypothetical protein | - |
| IN878_RS25050 | 35938..36183 | - | 246 | WP_065801591.1 | hypothetical protein | - |
| IN878_RS25055 | 36218..36634 | - | 417 | WP_023292131.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| IN878_RS25060 | 36631..36861 | - | 231 | WP_021312476.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| IN878_RS25065 | 36968..37129 | + | 162 | WP_203037272.1 | hypothetical protein | - |
| IN878_RS25070 | 37180..37440 | + | 261 | WP_032280099.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| IN878_RS25075 | 37475..37723 | + | 249 | WP_071787159.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| IN878_RS25080 | 37885..38826 | - | 942 | WP_058820814.1 | hypothetical protein | - |
| IN878_RS25085 | 39061..40461 | - | 1401 | WP_000996654.1 | ISNCY family transposase | - |
| IN878_RS25090 | 40738..41163 | + | 426 | WP_000422741.1 | IS66 family insertion sequence hypothetical protein | - |
| IN878_RS25095 | 41160..41510 | + | 351 | WP_000624722.1 | IS66 family insertion sequence element accessory protein TnpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | cseA / vat | 1..115346 | 115346 | |
| - | inside | IScluster/Tn | - | cseA | 10354..43940 | 33586 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 14975.40 Da Isoelectric Point: 8.5464
>T177928 WP_023292131.1 NZ_CP063337:c36634-36218 [Escherichia coli]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN
Download Length: 417 bp
>T177928 NZ_CP083768:c5167579-5167196 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8723.76 Da Isoelectric Point: 4.3410
>AT177928 WP_021312476.1 NZ_CP063337:c36861-36631 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL
Download Length: 231 bp
>AT177928 NZ_CP083768:c5167821-5167579 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6H2GRP8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6B7Q5S4 |