177926

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 12393..12858 Replicon plasmid pBS74R-D_3
Accession NZ_CP063335
Organism Escherichia coli strain BS74R-D

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag IN878_RS24675 Protein ID WP_001230707.1
Coordinates 12393..12644 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag IN878_RS24680 Protein ID WP_000801440.1
Coordinates 12631..12858 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN878_RS24645 7492..7725 + 234 Protein_8 hypothetical protein -
IN878_RS24650 7877..8404 - 528 WP_000577043.1 DUF2726 domain-containing protein -
IN878_RS24655 8539..8943 - 405 WP_012421203.1 hypothetical protein -
IN878_RS24660 8956..9820 - 865 Protein_11 replication initiation protein -
IN878_RS24665 11234..11749 + 516 WP_001025395.1 J domain-containing protein -
IN878_RS24670 11854..12396 + 543 WP_001083906.1 hypothetical protein -
IN878_RS24675 12393..12644 + 252 WP_001230707.1 hypothetical protein Toxin
IN878_RS24680 12631..12858 + 228 WP_000801440.1 hypothetical protein Antitoxin
IN878_RS24685 13312..13611 + 300 WP_000650309.1 hypothetical protein -
IN878_RS24690 13702..14052 + 351 WP_000854259.1 hypothetical protein -
IN878_RS24695 14357..14605 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
IN878_RS24700 14602..14949 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
IN878_RS24705 15310..15813 + 504 WP_000781813.1 hypothetical protein -
IN878_RS24710 15817..17046 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
IN878_RS24715 17123..17416 + 294 WP_000134733.1 LuxR family transcriptional regulator -
IN878_RS24720 17413..17628 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33851 33851


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T177926 WP_001230707.1 NZ_CP063335:12393-12644 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T177926 NZ_CP083768:c4686056-4685772 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTATGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT177926 WP_000801440.1 NZ_CP063335:12631-12858 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT177926 NZ_CP083768:c4686288-4686046 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References