Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 81871..82393 Replicon plasmid pBS74R-D_1
Accession NZ_CP063333
Organism Escherichia coli strain BS74R-D

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag IN878_RS23990 Protein ID WP_000220560.1
Coordinates 82112..82393 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag IN878_RS23985 Protein ID WP_000121743.1
Coordinates 81871..82122 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN878_RS23960 77542..77823 + 282 WP_001185482.1 hypothetical protein -
IN878_RS23965 77848..78825 + 978 WP_024245156.1 hypothetical protein -
IN878_RS23970 78822..79247 - 426 WP_000202942.1 hypothetical protein -
IN878_RS23975 79315..79761 - 447 Protein_93 hypothetical protein -
IN878_RS23980 79801..80637 - 837 WP_001050931.1 replication initiation protein -
IN878_RS23985 81871..82122 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
IN878_RS23990 82112..82393 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IN878_RS23995 82468..83165 + 698 Protein_97 IS1 family transposase -
IN878_RS24000 83175..83483 + 309 Protein_98 replication initiation protein -
IN878_RS24005 85071..86039 + 969 WP_030005799.1 IS5 family transposase -
IN878_RS24010 86179..86565 - 387 WP_001039464.1 hypothetical protein -
IN878_RS24015 87146..87328 + 183 Protein_101 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / qnrS1 / tet(M) / tet(A) / dfrA12 / aadA2 / sul3 / sul2 / floR - 1..109936 109936


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T177924 WP_000220560.1 NZ_CP063333:82112-82393 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T177924 NZ_CP083768:3975014-3975232 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT177924 WP_000121743.1 NZ_CP063333:81871-82122 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT177924 NZ_CP083768:3974613-3974987 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References