Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2037666..2038320 Replicon chromosome
Accession NZ_CP063332
Organism Escherichia coli strain BS74R-D

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IN878_RS09975 Protein ID WP_000244777.1
Coordinates 2037666..2038073 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IN878_RS09980 Protein ID WP_000354046.1
Coordinates 2038054..2038320 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN878_RS09955 2033623..2035356 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
IN878_RS09960 2035362..2036072 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IN878_RS09965 2036097..2036993 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IN878_RS09970 2037105..2037626 + 522 WP_001055874.1 flavodoxin FldB -
IN878_RS09975 2037666..2038073 - 408 WP_000244777.1 toxin CptA Toxin
IN878_RS09980 2038054..2038320 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IN878_RS09985 2038563..2039543 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IN878_RS09990 2039739..2040398 - 660 WP_000250274.1 hemolysin III family protein -
IN878_RS09995 2040562..2040873 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IN878_RS10000 2040918..2042351 + 1434 WP_001573179.1 6-phospho-beta-glucosidase BglA -
IN878_RS10005 2042408..2043151 - 744 WP_001570285.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T177914 WP_000244777.1 NZ_CP063332:c2038073-2037666 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T177914 NZ_CP083757:537051-537332 [Marinilongibacter aquaticus]
ATGAAAACTTCAAAATTTGACATAGCAGATTATTTGGACAGCAAAGAAATGATTGCAGAATATTTGAATACTGCTCTCGA
AAATGGAAATAATCAAGACGTTATTAACGCCATTGGACACATTGCGAAAGCCATTGGAATGACTAAAATCTCTGAAGAAA
CAGGATTAAGCAGACCTAGTTTATACAAAGCATTGTCAGACGGAGCAAAACCTCAATTTGCCACAATTATGAAAGTTTTG
AAAGCAATCGGTGGACAAATTCATATAAATCCGACAGCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT177914 WP_000354046.1 NZ_CP063332:c2038320-2038054 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT177914 NZ_CP083757:536746-537045 [Marinilongibacter aquaticus]
ATGTACTTTATTGAGAAAACAACAGAGTTTGACAAGTGGCTTAAAAAGCTAAAAGACATTAGAGCAAAAGCCAAAATAAT
TTTCAGAATCCAAAAAATTGAATCTGACGAACATTTTGGTGATTGTAAACCTGTTGGAGACGGTATTCGTGAACTGAGAA
TAAATTACGCTAAAGGATACCGAGTTTACTTTAAAGAAAAAGGTGATAAAATCATCATTTTACTTGTTGGTGGAGATAAA
TCGACTCAACAAAAAGACATTGAAAAAGCGAAAGAAATCTGGGAAAAATTAAACAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References