Detailed information of TA system TA177903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 614071..614597
• Replicon: chromosome
• Accession: NZ_CP063332
• Organism: Escherichia coli strain BS74R-D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IN878_RS03110
• Protein ID: WP_000323025.1
• Coordinates: 614071..614358 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IN878_RS03115
• Protein ID: WP_000534858.1
• Coordinates: 614358..614597 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IN878_RS03065	609095..609310	-	216	WP_000839590.1	phage lysis protein EssD	-
IN878_RS03070	610064..610279	-	216	WP_000066484.1	cold shock-like protein CspB	-
IN878_RS03075	610580..610792	+	213	WP_000087756.1	cold shock-like protein CspF	-
IN878_RS03080	610847..610936	+	90	WP_120795389.1	hypothetical protein	-
IN878_RS03085	611214..611966	-	753	WP_001047135.1	antitermination protein	-
IN878_RS03090	611980..613029	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
IN878_RS03095	613031..613309	-	279	WP_012304870.1	hypothetical protein	-
IN878_RS03100	613376..613627	-	252	WP_000980994.1	hypothetical protein	-
IN878_RS03105	613844..613999	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
IN878_RS03110	614071..614358	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IN878_RS03115	614358..614597	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IN878_RS03120	614622..614927	+	306	WP_001326990.1	hypothetical protein	-
IN878_RS03125	615130..615462	+	333	WP_001301033.1	protein FlxA	-
IN878_RS03130	615899..617212	-	1314	WP_000589005.1	ISNCY family transposase	-
IN878_RS03135	617981..618268	-	288	Protein_619	hypothetical protein	-
IN878_RS03140	618879..619235	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
IN878_RS03145	619232..619483	-	252	Protein_621	DUF977 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	599329..629497	30168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T177903 WP_000323025.1 NZ_CP063332:c614358-614071 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T177903 NZ_CP083754:c2196771-2196415 [Serratia marcescens]
ATGATCTTTTTGACCGCAGAGGATATTGCGGAGTTCAATGCTGAAATTGTGCCTCACGGCCGCCAGGAAGGCAGCAAAGT
TGAGGCTGTAGCCAACCGCGTGCTCAATGCTTACCACTACGAGAACGTCACAGACGTTTACCGCTTGGCCGCGCTCTATC
TCATCGCCATCAGCCACGGTCACATTTTTCTTGATGGCAACAAACGTACGGCCTTCCAAAGCATGGCGCTATTTCTCGGT
ATTAATGGCATTGCACTGCGGGAAGACGCAGAACTGGTCGAAATTACGGTTGAAGCAGCTCAAGGCCGCCTCAACGTTGA
ACAAGCCGCTGAACACTTACGCCGGCTAACCGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT177903 WP_000534858.1 NZ_CP063332:c614597-614358 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT177903 NZ_CP083754:c2196977-2196768 [Serratia marcescens]
ATGACTACGATTAGCTACACGGCGGCGAGAAACAATTTGGCCGAGGTGCTTTTGGAAGCGCAAAAACAGCCGGTGGAAGT
GACGCGCCGCGGACACGATGAGGTTTACATCATCAGTAAGGCCGACTATGAGGTTTTGGTCAAAGCCAAAGTGAAAGCGC
GCATTCAGGCTAAACACGCGGATACGATTAAGGCGCTGGCGAATAGATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8