Detailed information of TA system TA177899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 26440..27017
• Replicon: plasmid pLC-693_1
• Accession: NZ_CP063315
• Organism: Proteus vulgaris strain LC-693

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: IM703_RS18555
• Protein ID: WP_023159957.1
• Coordinates: 26685..27017 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IM703_RS18550
• Protein ID: WP_096864992.1
• Coordinates: 26440..26685 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM703_RS18515	22193..23239	+	1047	WP_072070749.1	ParB/RepB/Spo0J family partition protein	-
IM703_RS18520	23242..23586	+	345	WP_023159966.1	single-stranded DNA-binding protein	-
IM703_RS18525	23579..24001	+	423	WP_048821775.1	hypothetical protein	-
IM703_RS18530	24011..24403	+	393	WP_109910891.1	hypothetical protein	-
IM703_RS18535	24753..25121	+	369	WP_109910890.1	hypothetical protein	-
IM703_RS18540	25219..25446	-	228	WP_096864993.1	DNA partition complex ParG	-
IM703_RS18545	25547..26170	-	624	WP_109910889.1	AAA family ATPase	-
IM703_RS18550	26440..26685	+	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IM703_RS18555	26685..27017	+	333	WP_023159957.1	endoribonuclease MazF	Toxin
IM703_RS18560	27048..27428	+	381	WP_096864991.1	hypothetical protein	-
IM703_RS18565	27514..27657	-	144	WP_071547955.1	type I toxin-antitoxin system Hok family toxin	-
IM703_RS18570	28022..28819	-	798	WP_048607643.1	hypothetical protein	-
IM703_RS18575	29498..30178	+	681	WP_197270028.1	site-specific integrase	-
IM703_RS18580	30347..31006	+	660	WP_125894568.1	DUF4365 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / aadA2 / qacE / sul1 / dfrA14 / qnrA1 / armA / blaNDM-1	-	1..85309	85309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T177899 WP_023159957.1 NZ_CP063315:26685-27017 [Proteus vulgaris]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T177899 NZ_CP083753:c6161-5856 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT177899 WP_096864992.1 NZ_CP063315:26440-26685 [Proteus vulgaris]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT177899 NZ_CP083753:c6381-6163 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75