Detailed information of TA system TA177894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3052549..3054173
• Replicon: chromosome
• Accession: NZ_CP063310
• Organism: Eggerthella sp. HF-1101

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: GS424_RS12970
• Protein ID: WP_160942640.1
• Coordinates: 3052890..3054173 (+)
• Length: 428 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: GS424_RS12965
• Protein ID: WP_160942641.1
• Coordinates: 3052549..3052893 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS424_RS12950	3048704..3050155	-	1452	WP_160942644.1	M20/M25/M40 family metallo-hydrolase	-
GS424_RS12955	3050301..3051257	+	957	WP_160942643.1	DUF2268 domain-containing protein	-
GS424_RS12960	3051254..3052402	-	1149	WP_160942642.1	DUF3810 domain-containing protein	-
GS424_RS12965	3052549..3052893	+	345	WP_160942641.1	helix-turn-helix domain-containing protein	Antitoxin
GS424_RS12970	3052890..3054173	+	1284	WP_160942640.1	type II toxin-antitoxin system HipA family toxin	Toxin
GS424_RS12975	3054455..3055498	-	1044	WP_160942639.1	ribonucleotide-diphosphate reductase subunit beta	-
GS424_RS12980	3055643..3058180	-	2538	WP_160942638.1	ribonucleoside-diphosphate reductase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-53)

Sequences

Toxin

Protein

• Length: 428 a.a.
• Molecular weight: 47576.50 Da
• Isoelectric Point: 5.0993

>T177894 WP_160942640.1 NZ_CP063310:3052890-3054173 [Eggerthella sp. HF-1101]
MIAANVFLWGTRIGAVQQEDVDDIPVFNYDPDFTLSGIELSPLEMPLSGALYSFPTLNGESFHGLPGMLADSLPDKFGNA
LLDSYLAQHGRDYRRLSAVERLLYTGKRGMGALEYEPSYDRFARNDDVDIDALVGVANDVLNRREGMRETMDAKGLAHLV
SVGTSAGGARAKAVIAWNRTTNDVRSGQVAADPGYEHWLIKFDGVEKNKDKGDSYDRPAYTRIEFAYYTMAIAAGLEMSA
CELFEENGRRHFMTRRFDRTADGGKLHMQTLGALAHFDFNAPAAHSYEQAASVMRRLNLGQDSLEQLYLRMTFNVLARNQ
DDHVKNLSFLMDRRGTWSLAPAYDVTYANDPGNKWLSRHQMTVAGKAENIGLDDLLAAAKAMDVPRIRALELTEQVNGAV
REWRTFAQSADVPEAACEEIQRNLLTL

Nucleotide

• Length: 1284 bp

>T177894 NZ_CP083751:c4834723-4833806 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12558.33 Da
• Isoelectric Point: 10.7225

>AT177894 WP_160942641.1 NZ_CP063310:3052549-3052893 [Eggerthella sp. HF-1101]
MKLTGYENNTAVLSELAQRIRDQRLALGLTQAELAKKSGVSSRTVARLESGDGIALDGFLGILRSLGILRNIDLLVPEYR
MAPTEAFDGRQKRKRAASSKSAQPNKGWTWGDER

Nucleotide

• Length: 345 bp

>AT177894 NZ_CP083751:c4835487-4834729 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA