Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3334288..3334908 Replicon chromosome
Accession NZ_CP063303
Organism Yersinia pestis strain 14D

Toxin (Protein)


Gene name Hha Uniprot ID Q66DR5
Locus tag IM067_RS14795 Protein ID WP_002208622.1
Coordinates 3334705..3334908 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q66DR4
Locus tag IM067_RS14790 Protein ID WP_002218472.1
Coordinates 3334288..3334656 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM067_RS14775 (IM067_14755) 3332483..3332743 + 261 WP_002208617.1 type B 50S ribosomal protein L31 -
IM067_RS14780 (IM067_14760) 3332759..3332902 + 144 WP_002208618.1 type B 50S ribosomal protein L36 -
IM067_RS14785 (IM067_14765) 3333776..3334129 + 354 WP_002208619.1 hypothetical protein -
IM067_RS14790 (IM067_14770) 3334288..3334656 + 369 WP_002218472.1 Hha toxicity modulator TomB Antitoxin
IM067_RS14795 (IM067_14775) 3334705..3334908 + 204 WP_002208622.1 expression modulating protein YmoA Toxin
IM067_RS14805 (IM067_14785) 3335796..3336185 + 390 WP_002208623.1 MGMT family protein -
IM067_RS14810 (IM067_14790) 3336321..3336842 - 522 WP_002208624.1 YbaY family lipoprotein -
IM067_RS14815 (IM067_14795) 3337099..3337959 + 861 WP_002208625.1 acyl-CoA thioesterase II -
IM067_RS14820 (IM067_14800) 3338219..3339508 - 1290 WP_002228344.1 ammonium transporter AmtB -
IM067_RS14825 (IM067_14805) 3339548..3339886 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8063.31 Da        Isoelectric Point: 6.4573

>T177892 WP_002208622.1 NZ_CP063303:3334705-3334908 [Yersinia pestis]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Download         Length: 204 bp

>T177892 NZ_CP083751:c4575651-4575118 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14215.00 Da        Isoelectric Point: 4.6121

>AT177892 WP_002218472.1 NZ_CP063303:3334288-3334656 [Yersinia pestis]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWINDPTSAINLQLNDLIEHIASFVMSFKIKYPDGSQLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKEHLFRLFSGEYVCTLMKT

Download         Length: 369 bp

>AT177892 NZ_CP083751:c4575928-4575662 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2K5S

Antitoxin

Source ID Structure
AlphaFold DB Q66DR4

References