Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4698832..4699451 Replicon chromosome
Accession NZ_CP063301
Organism Klebsiella grimontii strain M5a1

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag INR80_RS22275 Protein ID WP_004099646.1
Coordinates 4699233..4699451 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag INR80_RS22270 Protein ID WP_004129911.1
Coordinates 4698832..4699206 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INR80_RS22260 4694001..4695194 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
INR80_RS22265 4695217..4698363 + 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit -
INR80_RS22270 4698832..4699206 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
INR80_RS22275 4699233..4699451 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
INR80_RS22280 4699613..4700179 + 567 WP_004136050.1 maltose O-acetyltransferase -
INR80_RS22285 4700306..4700776 + 471 WP_004136048.1 YlaC family protein -
INR80_RS22290 4700751..4702205 - 1455 WP_004136046.1 PLP-dependent aminotransferase family protein -
INR80_RS22295 4702306..4703004 + 699 WP_004136044.1 GNAT family N-acetyltransferase -
INR80_RS22300 4703001..4703141 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
INR80_RS22305 4703141..4703404 - 264 WP_004129897.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T177878 WP_004099646.1 NZ_CP063301:4699233-4699451 [Klebsiella grimontii]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T177878 NZ_CP083746:1743616-1743912 [Pseudomonas sp. p1(2021b)]
ATGGAAAAGAGAACGCCTCATTGCCCGCTGGAGCGGGTGAGAACACTGGTTGCTGCCGGCCGGGTAATGCCTACGGCGAG
TGCATTGAGGGGCGCTCTGGCCCTTGGGCTGGATTATCAAGCGATGCTCGAAGTGATCCAGTCGTTAGAGCGTAGGGACT
TCTTCAAGAGCATGACGAGCTTTGCCGATCACCGGATCTGGCAAGACGTATATCGTCCACGGACAGGCGTCGGCTACGTT
TACCTCAAATTGTCAGTGATCGACGACGTGCTCATCGTATCCTTCAAGGAGCTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT177878 WP_004129911.1 NZ_CP063301:4698832-4699206 [Klebsiella grimontii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT177878 NZ_CP083746:1743916-1744317 [Pseudomonas sp. p1(2021b)]
ATGAAATGTCCGATTTGCGGTGGAGCAAAACTTGTCCCGGGCACTCAGGACATGCCGTACCGTTACCAAGGCGCGTCGAC
CCTGATCGAGAAGGTGAGGGGTGATTACTGCCCAGCCTGTGGGGAGGCGGTGCTCAGCCATGGCGAAGCGCAGCGCGTCA
GCGACGTGATGTCGGCCTTCAATCGCCAAGTCAATGCCGCACGTGTCGATCCGGGTTTCATCACCGCAGTGCGCAAGAAA
TTCGACCTGGACCAGCGCGAAGCGGGTGAAATCTTCGGCGGTGGCGTCAACGCTTTTTCCCGATATGAAAACGGCAAGAC
CAGGCCGCCGGTCGCCCTGGTAAAACTGTTCAAGTTGCTGGACCGGCATCCTGAACTGTTCGAGGAAATCAGGACGGCGT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References