Detailed information of TA system TA177863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54331..54856
• Replicon: plasmid pMAR14456-IncFII
• Accession: NZ_CP063279
• Organism: Klebsiella pneumoniae strain MAR14-456

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: IL253_RS28030
• Protein ID: WP_004197633.1
• Coordinates: 54551..54856 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: IL253_RS28025
• Protein ID: WP_004197642.1
• Coordinates: 54331..54549 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IL253_RS27980	49729..50052	+	324	WP_019725650.1	hypothetical protein	-
IL253_RS27985	50359..51055	+	697	Protein_56	IS1 family transposase	-
IL253_RS27990	51262..51456	+	195	WP_019725033.1	hypothetical protein	-
IL253_RS27995	51500..51730	-	231	WP_019725034.1	hypothetical protein	-
IL253_RS28000	51744..51947	-	204	WP_019725040.1	hemolysin expression modulator Hha	-
IL253_RS28005	51981..52349	-	369	WP_015065502.1	hypothetical protein	-
IL253_RS28010	52393..52887	-	495	WP_009310053.1	hypothetical protein	-
IL253_RS28015	52918..53490	-	573	WP_015065504.1	hypothetical protein	-
IL253_RS28020	53487..53735	-	249	WP_019725036.1	hypothetical protein	-
IL253_RS28025	54331..54549	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
IL253_RS28030	54551..54856	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
IL253_RS28035	55025..55426	+	402	WP_072199347.1	hypothetical protein	-
IL253_RS28040	55453..55776	+	324	WP_004197641.1	hypothetical protein	-
IL253_RS28045	55773..56789	+	1017	WP_004197639.1	hypothetical protein	-
IL253_RS28050	56987..57781	+	795	WP_004197635.1	site-specific integrase	-
IL253_RS28055	58221..58400	-	180	WP_006788217.1	hypothetical protein	-
IL253_RS28060	58520..59146	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / tet(A) / qnrB1 / blaCTX-M-15 / dfrA14 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..127014	127014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T177863 WP_004197633.1 NZ_CP063279:54551-54856 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T177863 NZ_CP083731:4203773-4204189 [Salmonella sp. A29-2]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCTGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT177863 WP_004197642.1 NZ_CP063279:54331-54549 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT177863 NZ_CP083731:4203546-4203776 [Salmonella sp. A29-2]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5