Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 104548..105458 Replicon plasmid pMAR14456-IncHI1B
Accession NZ_CP063278
Organism Klebsiella pneumoniae strain MAR14-456

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag IL253_RS27010 Protein ID WP_004026354.1
Coordinates 104548..105018 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag IL253_RS27015 Protein ID WP_004026357.1
Coordinates 105015..105458 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL253_RS26990 99960..100358 - 399 WP_004212796.1 H-NS histone family protein -
IL253_RS26995 100695..101189 - 495 WP_004212794.1 thermonuclease family protein -
IL253_RS27000 101367..102316 + 950 Protein_107 DUF1173 family protein -
IL253_RS27005 103702..104399 - 698 Protein_108 IS1 family transposase -
IL253_RS27010 104548..105018 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
IL253_RS27015 105015..105458 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
IL253_RS27020 105559..106062 - 504 Protein_111 DUF4113 domain-containing protein -
IL253_RS27025 106160..106381 - 222 WP_004213643.1 DUF2188 domain-containing protein -
IL253_RS27030 106526..106681 - 156 WP_004213642.1 hypothetical protein -
IL253_RS27035 106756..108882 - 2127 WP_143441901.1 TonB-dependent receptor family protein -
IL253_RS27040 108969..109922 - 954 Protein_115 fec operon regulator FecR -
IL253_RS27045 109919..110440 - 522 WP_004213635.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..232362 232362
- flank IS/Tn - - 103702..104079 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T177861 WP_004026354.1 NZ_CP063278:c105018-104548 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T177861 NZ_CP083731:3600812-3601069 [Salmonella sp. A29-2]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT177861 WP_004026357.1 NZ_CP063278:c105458-105015 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT177861 NZ_CP083731:c3600987-3600655 [Salmonella sp. A29-2]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGG
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTTAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References