Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 85991..86616 Replicon chromosome
Accession NZ_CP063277
Organism Klebsiella pneumoniae strain MAR14-456

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag IL253_RS00420 Protein ID WP_012737091.1
Coordinates 85991..86374 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IL253_RS00425 Protein ID WP_004150355.1
Coordinates 86374..86616 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IL253_RS00405 83357..84259 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IL253_RS00410 84256..84891 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IL253_RS00415 84888..85817 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IL253_RS00420 85991..86374 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
IL253_RS00425 86374..86616 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
IL253_RS00430 86821..87738 + 918 WP_012737090.1 alpha/beta hydrolase -
IL253_RS00435 87752..88693 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IL253_RS00440 88738..89175 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
IL253_RS00445 89172..90032 - 861 WP_004146232.1 virulence factor BrkB family protein -
IL253_RS00450 90026..90625 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T177844 WP_012737091.1 NZ_CP063277:c86374-85991 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T177844 NZ_CP083728:18831-19007 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT177844 WP_004150355.1 NZ_CP063277:c86616-86374 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT177844 NZ_CP083728:c19169-18995 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References