Detailed information of TA system TA177843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1257124..1258040
• Replicon: chromosome
• Accession: NZ_CP063276
• Organism: Bacillus mojavensis strain LDFZ001

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A7G7U9Y2
• Locus tag: IM743_RS06475
• Protein ID: WP_024121091.1
• Coordinates: 1257294..1258040 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A7G7U9Y3
• Locus tag: IM743_RS06470
• Protein ID: WP_024121090.1
• Coordinates: 1257124..1257294 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM743_RS06435	1253987..1254316	+	330	WP_106020448.1	XkdW family protein	-
IM743_RS06440	1254313..1254477	+	165	WP_193660423.1	XkdX family protein	-
IM743_RS06445	1254521..1255360	+	840	WP_193660424.1	phage portal protein	-
IM743_RS06450	1255416..1255685	+	270	WP_059292937.1	hemolysin XhlA family protein	-
IM743_RS06455	1255697..1255960	+	264	WP_059352788.1	phage holin	-
IM743_RS06460	1255973..1256866	+	894	WP_059336101.1	N-acetylmuramoyl-L-alanine amidase	-
IM743_RS06465	1256903..1257040	-	138	WP_125825426.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
IM743_RS06470	1257124..1257294	-	171	WP_024121090.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
IM743_RS06475	1257294..1258040	-	747	WP_024121091.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
IM743_RS06480	1258150..1259151	-	1002	WP_193660425.1	inorganic phosphate transporter	-
IM743_RS06485	1259164..1259781	-	618	WP_193660426.1	DUF47 domain-containing protein	-
IM743_RS06490	1260057..1261373	-	1317	WP_193660427.1	serine/threonine exchanger	-
IM743_RS06495	1261768..1262718	+	951	WP_193660428.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29076.48 Da
• Isoelectric Point: 4.4986

>T177843 WP_024121091.1 NZ_CP063276:c1258040-1257294 [Bacillus mojavensis]
MLLFFQIMVWCTMAALGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMIYWTYDPSSLFTNWERYVIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLDRLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CHYSTQSDKDRLTEHMEDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQSYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T177843 NZ_CP083721:c1024184-1023888 [Bradyrhizobium sp. 41S5]
GTGAAGCTCGTCTGGACGCGCTTCGCACTGTCCGATCGCGACGACATTTTCAGCTACATCGAGGCCGAGAATCCCCGTGC
CGCGGTTCATGTCGATGAACAGATCGCCGATGCCGCGCGGCGCCTGCTCGACTTTCCTGACAGTGGCCGGCCCGGGCGTG
TCGCCGGCACGCGGGAGTTAGTCATCCCGCGCACACCTTATGTCGCAGCGTACCTTGTCGACGGCGAGACCGTTCGCATC
TTGCGCGTGCTTCACGGCGCGCAGATGTGGCCCGACGAACTTGCAAATGACGATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6578.62 Da
• Isoelectric Point: 10.9236

>AT177843 WP_024121090.1 NZ_CP063276:c1257294-1257124 [Bacillus mojavensis]
MENAFQNGCHPRAKKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT177843 NZ_CP083721:c1024489-1024181 [Bradyrhizobium sp. 41S5]
ATGGCTTCGAATGCCCTGGTCCAAACCCGCATCGATGCGGACGTAAAAGAGAAGGCCACCGCGGTTCTGGAAAACATGGG
TCTCACGGTGTCGGACGCGGTGCGGATATTGCTGACGCGCACGGCCAATGAAGGGACGCTTCCACTGGAGCTCGTCAGCA
ACAGCCAAGCTTACGACAGCTGGTTTCGCGAGAAGGTGCGCCAAGCGCTGGCGGATACACGGCCCAGTCTCGATGATTCC
GAAGTCGACGCGCATTTCGCGCAACGTCGTGCCGCCGCACTTCGAAAAGCGGCGGAACGCAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A7G7U9Y2

Antitoxin

• AlphaFold DB: A0A7G7U9Y3