Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 454576..455212 Replicon chromosome
Accession NZ_CP063276
Organism Bacillus mojavensis strain LDFZ001

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag IM743_RS02190 Protein ID WP_003156187.1
Coordinates 454862..455212 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag IM743_RS02185 Protein ID WP_003225183.1
Coordinates 454576..454857 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM743_RS02165 450933..451532 - 600 WP_044161737.1 rhomboid family intramembrane serine protease -
IM743_RS02170 451627..451992 + 366 WP_044161735.1 holo-ACP synthase -
IM743_RS02175 452158..453174 + 1017 WP_082687822.1 outer membrane lipoprotein carrier protein LolA -
IM743_RS02180 453291..454460 + 1170 WP_044161733.1 alanine racemase -
IM743_RS02185 454576..454857 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
IM743_RS02190 454862..455212 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IM743_RS02195 455328..456152 + 825 WP_082687807.1 RsbT co-antagonist protein RsbRA -
IM743_RS02200 456157..456522 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
IM743_RS02205 456526..456927 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
IM743_RS02210 456939..457946 + 1008 WP_193660136.1 phosphoserine phosphatase RsbU -
IM743_RS02215 458007..458336 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
IM743_RS02220 458333..458815 + 483 WP_024120302.1 anti-sigma B factor RsbW -
IM743_RS02225 458781..459569 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
IM743_RS02230 459569..460168 + 600 WP_044161719.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T177842 WP_003156187.1 NZ_CP063276:454862-455212 [Bacillus mojavensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T177842 NZ_CP083719:c1216537-1216358 [Cupriavidus metallidurans]
TTGCATTCGTCCACGCTGATCCGATTGCTCGAACAGAAGGGGTGGAAGCTGGTGCGCACGCATGGCAGTCATTTCACCTT
CAAACATCCGCGGCACATGCTGATTGTGACCGTGCCCCATCCGAAGAAGGATCTGCCGACAGGCACGGTCCGAAACATTC
TGAGGACGGCCGGACTATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT177842 WP_003225183.1 NZ_CP063276:454576-454857 [Bacillus mojavensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT177842 NZ_CP083719:c1216328-1215924 [Cupriavidus metallidurans]
ATGGACTTTCCCATTGCCATTCACAAGGATGCGAACTCGGTCTACGGCGTAACGGTGCCCGATGTGGCGGGCTGTTACTC
CGCCGGCGAATCGATCGAGGAAGCGGTCCGCAACGCACGCGAGGCCATCACGACTCACGTGGTGGCCCTGCTCTCCCTTG
GCGAGGAGGTTGTTTTGAAAACGACGCCGATCGGCACGCTGATGGCTCAACCTGAGTACGCAGACGCGATCTGGGCGCTG
GCCGACATCGACATGAGCAAGCTCGACGCACGCCCGGAGCGAATCAATATCAGCCTGCCTCGTTTCGTATTGCACAAGAT
CGACATGTTCGTGGAACGCCGCCACGAAACCCGGAGCGGGTTCCTCGCGCGCGTCGCGCTGGACGCCATCGCGGGCAGCG
TCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References